Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids
Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from c...
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4354 |
| _version_ | 1855032106669834240 |
|---|---|
| author | Perez, Rosa Mas, O. Navarro, Luis Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Mas, O. Navarro, Luis Perez, Rosa |
| author_facet | Perez, Rosa Mas, O. Navarro, Luis Durán-Vila, Núria |
| author_sort | Perez, Rosa |
| collection | ReDivia |
| description | Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis. |
| format | article |
| id | ReDivia4354 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43542025-04-25T14:42:56Z Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids Perez, Rosa Mas, O. Navarro, Luis Durán-Vila, Núria Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis. 2017-06-01T10:09:55Z 2017-06-01T10:09:55Z 1999 article Perez, R., Mas, O., Navarro, L.& Duran-Vila, N. (1999). Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids. Plant Cell Tissue and Organ Culture, 55(1), 71-74. 0167-6857 http://hdl.handle.net/20.500.11939/4354 10.1023/A:1026433405701 en openAccess Impreso |
| spellingShingle | Perez, Rosa Mas, O. Navarro, Luis Durán-Vila, Núria Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title | Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title_full | Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title_fullStr | Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title_full_unstemmed | Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title_short | Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| title_sort | production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids |
| url | http://hdl.handle.net/20.500.11939/4354 |
| work_keys_str_mv | AT perezrosa productionandcryoconservationofembryogenicculturesofmandarinandmandarinhybrids AT maso productionandcryoconservationofembryogenicculturesofmandarinandmandarinhybrids AT navarroluis productionandcryoconservationofembryogenicculturesofmandarinandmandarinhybrids AT duranvilanuria productionandcryoconservationofembryogenicculturesofmandarinandmandarinhybrids |