Detection of Double-Stranded-Rna by Elisa and Dot Immunobinding Assay using an Antiserum to Synthetic Polynucleotides
An antiserum against polyinosinic-polycytidylic acid (In-Cn) was used to detect double-stranded RNA (dsRNA) by several serological techniques. DsRNA was readily detected by indirect ELISA (ELISA-I) and dot immunobinding assay (DIA). Addition of the antigen to poly-L-lysine-precoated plates and block...
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4339 |
Ejemplares similares: Detection of Double-Stranded-Rna by Elisa and Dot Immunobinding Assay using an Antiserum to Synthetic Polynucleotides
- Interference Between Citrus Tristeza Virus (CTV) Isolates Detected by Analysis of Double Stranded RNA (dsRNA)
- Segregation of Citrus Tristeza Virus Strains Evidenced by Double Stranded RNA (dsRNA) Analysis
- Variations in Pathogenicity and Double-Stranded RNA (dsRNA) Patterns of Citrus Tristeza Virus Isolate Induced by Host Passage
- Suitable Conditions for Detecting Apple Chlorotic Leaf-Spot Virus in Apricot Trees by Enzyme-Linked Immunosorbent-Assay (Elisa)
- Double antigen sandwich ELISA for detection of antibodies against Babesia bovis
- Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs