Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants
Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and beta-glucuronidase (GUS) genes wa...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4333 |
| _version_ | 1855491734530686976 |
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| author | Pena, Leandro Cervera, Magdalena Juárez, José Navarro, Antonio Pina, José A. Durán-Vila, Núria Navarro, Luis |
| author_browse | Cervera, Magdalena Durán-Vila, Núria Juárez, José Navarro, Antonio Navarro, Luis Pena, Leandro Pina, José A. |
| author_facet | Pena, Leandro Cervera, Magdalena Juárez, José Navarro, Antonio Pina, José A. Durán-Vila, Núria Navarro, Luis |
| author_sort | Pena, Leandro |
| collection | ReDivia |
| description | Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and beta-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus. |
| format | Artículo |
| id | ReDivia4333 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43332025-04-25T14:42:52Z Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants Pena, Leandro Cervera, Magdalena Juárez, José Navarro, Antonio Pina, José A. Durán-Vila, Núria Navarro, Luis Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and beta-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus. 2017-06-01T10:09:52Z 2017-06-01T10:09:52Z 1995 JUL article PENA, L., CERVERA, M., JUAREZ, J., NAVARRO, A., PINA, JA, DURANVILA, N., Navarro, L. (1995). Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants. Plant Cell Reports, 14(10), 616-619. 0721-7714 http://hdl.handle.net/20.500.11939/4333 10.1007/BF00232724 en openAccess Impreso |
| spellingShingle | Pena, Leandro Cervera, Magdalena Juárez, José Navarro, Antonio Pina, José A. Durán-Vila, Núria Navarro, Luis Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title | Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title_full | Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title_fullStr | Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title_full_unstemmed | Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title_short | Agrobacterium-Mediated Transformation of Sweet Orange and Regeneration of Transgenic Plants |
| title_sort | agrobacterium mediated transformation of sweet orange and regeneration of transgenic plants |
| url | http://hdl.handle.net/20.500.11939/4333 |
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