The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance
The avirulence determinant triggering the resistance conferred by the tomato gene Sw-5 against Tomato spotted wilt virus (TSWV) is still unresolved. Sequence comparison showed two substitutions (C118Y and T120N) in the movement protein NSm present only in TSWV resistance-breaking (RB) isolates. In t...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4324 |
| _version_ | 1855032101534957568 |
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| author | Peiro, Ana Canizares, M. C. Rubio, Luis Lopez, Carmelo Moriones, Enrique Aramburu, Jose Sánchez-Navarro, Jesús A. |
| author_browse | Aramburu, Jose Canizares, M. C. Lopez, Carmelo Moriones, Enrique Peiro, Ana Rubio, Luis Sánchez-Navarro, Jesús A. |
| author_facet | Peiro, Ana Canizares, M. C. Rubio, Luis Lopez, Carmelo Moriones, Enrique Aramburu, Jose Sánchez-Navarro, Jesús A. |
| author_sort | Peiro, Ana |
| collection | ReDivia |
| description | The avirulence determinant triggering the resistance conferred by the tomato gene Sw-5 against Tomato spotted wilt virus (TSWV) is still unresolved. Sequence comparison showed two substitutions (C118Y and T120N) in the movement protein NSm present only in TSWV resistance-breaking (RB) isolates. In this work, transient expression of NSm of three TSWV isolates [RB1 (T120N), RB2 (C118Y) and non-resistance-breaking (NRB)] in Nicotiana benthamiana expressing Sw-5 showed a hypersensitive response (HR) only with NRB. Exchange of the movement protein of Alfalfa mosaic virus (AMV) with NSm supported cell-to-cell and systemic transport of the chimeric AMV RNAs into N.tabacum with or without Sw-5, except for the constructs with NBR when Sw-5 was expressed, although RB2 showed reduced cell-to-cell transport. Mutational analysis revealed that N120 was sufficient to avoid the HR, but the substitution V130I was required for systemic transport. Finally, co-inoculation of RB and NRB AMV chimeric constructs showed different prevalence of RB or NBR depending on the presence or absence of Sw-5. These results indicate that NSm is the avirulence determinant for Sw-5 resistance, and mutations C118Y and T120N are responsible for resistance breakdown and have a fitness penalty in the context of the heterologous AMV system. |
| format | article |
| id | ReDivia4324 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43242025-04-25T14:42:51Z The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance Peiro, Ana Canizares, M. C. Rubio, Luis Lopez, Carmelo Moriones, Enrique Aramburu, Jose Sánchez-Navarro, Jesús A. The avirulence determinant triggering the resistance conferred by the tomato gene Sw-5 against Tomato spotted wilt virus (TSWV) is still unresolved. Sequence comparison showed two substitutions (C118Y and T120N) in the movement protein NSm present only in TSWV resistance-breaking (RB) isolates. In this work, transient expression of NSm of three TSWV isolates [RB1 (T120N), RB2 (C118Y) and non-resistance-breaking (NRB)] in Nicotiana benthamiana expressing Sw-5 showed a hypersensitive response (HR) only with NRB. Exchange of the movement protein of Alfalfa mosaic virus (AMV) with NSm supported cell-to-cell and systemic transport of the chimeric AMV RNAs into N.tabacum with or without Sw-5, except for the constructs with NBR when Sw-5 was expressed, although RB2 showed reduced cell-to-cell transport. Mutational analysis revealed that N120 was sufficient to avoid the HR, but the substitution V130I was required for systemic transport. Finally, co-inoculation of RB and NRB AMV chimeric constructs showed different prevalence of RB or NBR depending on the presence or absence of Sw-5. These results indicate that NSm is the avirulence determinant for Sw-5 resistance, and mutations C118Y and T120N are responsible for resistance breakdown and have a fitness penalty in the context of the heterologous AMV system. 2017-06-01T10:09:51Z 2017-06-01T10:09:51Z 2014 OCT 2014 article publishedVersion Peiro, A., Canizares, M. C., Rubio, L., Lopez, C., Moriones, E., Aramburu, J. et al. (2014). The movement protein (NSm), of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance. Molecular Plant Pathology, 15(8), 802-813. 1464-6722 http://hdl.handle.net/20.500.11939/4324 10.1111/mpp.12142 en openAccess Impreso |
| spellingShingle | Peiro, Ana Canizares, M. C. Rubio, Luis Lopez, Carmelo Moriones, Enrique Aramburu, Jose Sánchez-Navarro, Jesús A. The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title | The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title_full | The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title_fullStr | The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title_full_unstemmed | The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title_short | The movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance |
| title_sort | movement protein nsm of tomato spotted wilt virus is the avirulence determinant in the tomato sw 5 gene based resistance |
| url | http://hdl.handle.net/20.500.11939/4324 |
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