Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions

Cucumber mosaic virus, Tomato spotted wilt virus, Tomato mosaic virus, Tomato chlorosis virus, Pepino mosaic virus, Torrado tomato virus and Tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. The early detection of these pathogens is ess...

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Autores principales: Panno, Stefano, Davino, Salvatore, Rubio, Luis, Rangel, Ezequiel A., Davino, Mario, Garcia-Hernandez, Jorge, Olmos, Antonio
Formato: article
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/4314
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author Panno, Stefano
Davino, Salvatore
Rubio, Luis
Rangel, Ezequiel A.
Davino, Mario
Garcia-Hernandez, Jorge
Olmos, Antonio
author_browse Davino, Mario
Davino, Salvatore
Garcia-Hernandez, Jorge
Olmos, Antonio
Panno, Stefano
Rangel, Ezequiel A.
Rubio, Luis
author_facet Panno, Stefano
Davino, Salvatore
Rubio, Luis
Rangel, Ezequiel A.
Davino, Mario
Garcia-Hernandez, Jorge
Olmos, Antonio
author_sort Panno, Stefano
collection ReDivia
description Cucumber mosaic virus, Tomato spotted wilt virus, Tomato mosaic virus, Tomato chlorosis virus, Pepino mosaic virus, Torrado tomato virus and Tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. The early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. In this study, a procedure based on two multiplex RT-PCRs was developed for the sensitive and reliable detection of these seven viruses. Serial dilutions of positive controls were analysed by this methodology, and the results were compared with those obtained by ELISA and singleplex versions of RT-PCR. The multiplex and singleplex RT-PCR assays were able to detect specific targets at the same dilution and were 100 times more sensitive than ELISA. The multiplex versions were able to detect composite samples containing different concentrations of specific targets at ratios from 1:1 to 1:1000. In addition, 45 symptomatic tomato samples collected in different tomato-growing areas of Sicily (Italy) were analysed by multiplex RT-PCR, singleplex RT-PCR and commercially available ELISA tests. Similar results were obtained using the RT-PCR techniques, with a higher sensitivity than ELISA, revealing a common occurrence of mixed infections and confirming the presence of these seven virus species in Italy. (C) 2012 Elsevier B.V. All rights reserved.
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spelling ReDivia43142025-04-25T14:42:48Z Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions Panno, Stefano Davino, Salvatore Rubio, Luis Rangel, Ezequiel A. Davino, Mario Garcia-Hernandez, Jorge Olmos, Antonio Cucumber mosaic virus, Tomato spotted wilt virus, Tomato mosaic virus, Tomato chlorosis virus, Pepino mosaic virus, Torrado tomato virus and Tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. The early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. In this study, a procedure based on two multiplex RT-PCRs was developed for the sensitive and reliable detection of these seven viruses. Serial dilutions of positive controls were analysed by this methodology, and the results were compared with those obtained by ELISA and singleplex versions of RT-PCR. The multiplex and singleplex RT-PCR assays were able to detect specific targets at the same dilution and were 100 times more sensitive than ELISA. The multiplex versions were able to detect composite samples containing different concentrations of specific targets at ratios from 1:1 to 1:1000. In addition, 45 symptomatic tomato samples collected in different tomato-growing areas of Sicily (Italy) were analysed by multiplex RT-PCR, singleplex RT-PCR and commercially available ELISA tests. Similar results were obtained using the RT-PCR techniques, with a higher sensitivity than ELISA, revealing a common occurrence of mixed infections and confirming the presence of these seven virus species in Italy. (C) 2012 Elsevier B.V. All rights reserved. 2017-06-01T10:09:50Z 2017-06-01T10:09:50Z 2012 DEC 2012 article acceptedVersion Panno, Stefano, Davino, Salvatore, Rubio, L., Rangel, Ezequiel, Davino, Mario, Garcia-Hernandez, J., Olmos, A. (2012). Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions. Journal of virological methods, 186(1-2), 152-156. 0166-0934 http://hdl.handle.net/20.500.11939/4314 10.1016/j.jviromet.2012.08.003 en openAccess Impreso
spellingShingle Panno, Stefano
Davino, Salvatore
Rubio, Luis
Rangel, Ezequiel A.
Davino, Mario
Garcia-Hernandez, Jorge
Olmos, Antonio
Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title_full Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title_fullStr Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title_full_unstemmed Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title_short Simultaneous detection of the seven main tomato-infecting RNA viruses by two multiplex reverse transcription polymerase chain reactions
title_sort simultaneous detection of the seven main tomato infecting rna viruses by two multiplex reverse transcription polymerase chain reactions
url http://hdl.handle.net/20.500.11939/4314
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