Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples
Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation w...
| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4288 |
| _version_ | 1855032095916687360 |
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| author | Palacio-Bielsa, Ana Lopez-Soriano, Pablo Buehlmann, Andreas van Doom, Joop Pham, Khanh Cambra, Miguel A. Berruete, Isabel M. Pothier, Joel F. Duffy, Brion Olmos, Antonio López, María M. |
| author_browse | Berruete, Isabel M. Buehlmann, Andreas Cambra, Miguel A. Duffy, Brion Lopez-Soriano, Pablo López, María M. Olmos, Antonio Palacio-Bielsa, Ana Pham, Khanh Pothier, Joel F. van Doom, Joop |
| author_facet | Palacio-Bielsa, Ana Lopez-Soriano, Pablo Buehlmann, Andreas van Doom, Joop Pham, Khanh Cambra, Miguel A. Berruete, Isabel M. Pothier, Joel F. Duffy, Brion Olmos, Antonio López, María M. |
| author_sort | Palacio-Bielsa, Ana |
| collection | ReDivia |
| description | Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. |
| format | article |
| id | ReDivia4288 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42882025-04-25T14:42:45Z Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples Palacio-Bielsa, Ana Lopez-Soriano, Pablo Buehlmann, Andreas van Doom, Joop Pham, Khanh Cambra, Miguel A. Berruete, Isabel M. Pothier, Joel F. Duffy, Brion Olmos, Antonio López, María M. Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. 2017-06-01T10:09:47Z 2017-06-01T10:09:47Z 2015 MAY 2015 article Palacio-Bielsa, A., Lopez-Soriano, P., Buehlmann, A., van Doom, J., Pham, K., Cambra, M. A., et al. (2015). Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples. Journal of microbiological methods, 112, 36-39. 0167-7012 http://hdl.handle.net/20.500.11939/4288 10.1016/j.mimet.2015.03.005 en openAccess Impreso |
| spellingShingle | Palacio-Bielsa, Ana Lopez-Soriano, Pablo Buehlmann, Andreas van Doom, Joop Pham, Khanh Cambra, Miguel A. Berruete, Isabel M. Pothier, Joel F. Duffy, Brion Olmos, Antonio López, María M. Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title_full | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title_fullStr | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title_full_unstemmed | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title_short | Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples |
| title_sort | evaluation of a real time pcr and a loop mediated isothermal amplification for detection of xanthomonas arboricola pv pruni in plant tissue samples |
| url | http://hdl.handle.net/20.500.11939/4288 |
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