Characterization of citrus HSVd isolates
Seven citrus isolates of Hop stunt viroid (HSVd) were subjected to retrotranscription and DNA amplification (RT-PCR), cloning and sequencing. Single stranded polymorphism (SSCP) analysis demonstrated the existence of variability among and within cachexia inducing sources of HSVd. The electrophoretic...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4284 |
| _version_ | 1855032095061049344 |
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| author | Palacio-Bielsa, Ana Romero-Durbán, J. Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Palacio-Bielsa, Ana Romero-Durbán, J. |
| author_facet | Palacio-Bielsa, Ana Romero-Durbán, J. Durán-Vila, Núria |
| author_sort | Palacio-Bielsa, Ana |
| collection | ReDivia |
| description | Seven citrus isolates of Hop stunt viroid (HSVd) were subjected to retrotranscription and DNA amplification (RT-PCR), cloning and sequencing. Single stranded polymorphism (SSCP) analysis demonstrated the existence of variability among and within cachexia inducing sources of HSVd. The electrophoretic profiles of SSCP appeared to be able to discriminate between non-cachexia and cachexia sources of HSVd. Sequence analysis demonstrated that the variable (V) domain was very conserved among the cachexia variants. Five nucleotide differences, affecting both the upper (3 nucleotides) and the lower (2 nucleotides) strands of the V domain, were identified as a motif discriminating cachexia and non-cachexia sequences. These five nucleotides affect the organization of a short helical region and two flanking loops of the V domain probably modifying the three-dimensional geometry of the molecule. The stability of the minimum free energy rod-like conformation of the cachexia sequences is lower than the non-cachexia. Information regarding the host effect on the evolution and variability of viroid quasispecies is also provided. |
| format | article |
| id | ReDivia4284 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42842025-04-25T14:42:44Z Characterization of citrus HSVd isolates Palacio-Bielsa, Ana Romero-Durbán, J. Durán-Vila, Núria Seven citrus isolates of Hop stunt viroid (HSVd) were subjected to retrotranscription and DNA amplification (RT-PCR), cloning and sequencing. Single stranded polymorphism (SSCP) analysis demonstrated the existence of variability among and within cachexia inducing sources of HSVd. The electrophoretic profiles of SSCP appeared to be able to discriminate between non-cachexia and cachexia sources of HSVd. Sequence analysis demonstrated that the variable (V) domain was very conserved among the cachexia variants. Five nucleotide differences, affecting both the upper (3 nucleotides) and the lower (2 nucleotides) strands of the V domain, were identified as a motif discriminating cachexia and non-cachexia sequences. These five nucleotides affect the organization of a short helical region and two flanking loops of the V domain probably modifying the three-dimensional geometry of the molecule. The stability of the minimum free energy rod-like conformation of the cachexia sequences is lower than the non-cachexia. Information regarding the host effect on the evolution and variability of viroid quasispecies is also provided. 2017-06-01T10:09:46Z 2017-06-01T10:09:46Z 2004 MAR 2004 article Palacio-Bielsa, A., Romero-Durbaan, J., Duran-Vila, N. (2004). Characterization of citrus HSVd isolates. Archives of Virology, 149(3), 537-552. 0304-8608 http://hdl.handle.net/20.500.11939/4284 10.1007/s00705-003-0223-5 en openAccess Impreso |
| spellingShingle | Palacio-Bielsa, Ana Romero-Durbán, J. Durán-Vila, Núria Characterization of citrus HSVd isolates |
| title | Characterization of citrus HSVd isolates |
| title_full | Characterization of citrus HSVd isolates |
| title_fullStr | Characterization of citrus HSVd isolates |
| title_full_unstemmed | Characterization of citrus HSVd isolates |
| title_short | Characterization of citrus HSVd isolates |
| title_sort | characterization of citrus hsvd isolates |
| url | http://hdl.handle.net/20.500.11939/4284 |
| work_keys_str_mv | AT palaciobielsaana characterizationofcitrushsvdisolates AT romerodurbanj characterizationofcitrushsvdisolates AT duranvilanuria characterizationofcitrushsvdisolates |