Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours
Fifty-six tumorigenic Spanish grapevine strains of Agrobacterium spp. were tested for biovar classification, pathogenicity on several hosts, opine utilization, 16S rRNA gene sequencing and PCR amplifications using five primer sets targeting chromosomal and Ti plasmid genes. Fifty of them belonged to...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4282 |
| _version_ | 1855032094669930496 |
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| author | Palacio-Bielsa, Ana González-Abolafio, Raquel Álvarez, Belén Lastra, Beatriz Cambra, Miguel A. Salcedo, Carmina I. López, María M. Penyalver, Ramón |
| author_browse | Cambra, Miguel A. González-Abolafio, Raquel Lastra, Beatriz López, María M. Palacio-Bielsa, Ana Penyalver, Ramón Salcedo, Carmina I. Álvarez, Belén |
| author_facet | Palacio-Bielsa, Ana González-Abolafio, Raquel Álvarez, Belén Lastra, Beatriz Cambra, Miguel A. Salcedo, Carmina I. López, María M. Penyalver, Ramón |
| author_sort | Palacio-Bielsa, Ana |
| collection | ReDivia |
| description | Fifty-six tumorigenic Spanish grapevine strains of Agrobacterium spp. were tested for biovar classification, pathogenicity on several hosts, opine utilization, 16S rRNA gene sequencing and PCR amplifications using five primer sets targeting chromosomal and Ti plasmid genes. Fifty of them belonged to A. vitis (biovar 3), three to A. tumefaciens (biovar 1) and three to A. rhizogenes (biovar 2). All strains were tumorigenic on grapevines. Most A. vitis strains were also pathogenic on tomato and tobacco plants, while the three A. tumefaciens strains were only pathogenic on grapevine. Although most A. vitis strains used octopine, 12 utilized neither octopine nor nopaline. 16S rRNA gene sequencing clearly distinguished between strains belonging to the three species. Those of A. vitis could be further divided into three chromosomal backgrounds according to their 16S ribosomal RNA gene sequences. No universal primer pair was found for the detection of all three Agrobacterium species isolated from grapevine. DNA from all A. vitis strains was amplified with the chromosomally-encoded pehA primer pair. In both A. vitis and A. tumefaciens a correlation was observed between the amplifications obtained using the tmr and the virA Ti-plasmid-targeting primer pairs. Three types of Ti plasmid were found in A. vitis strains according to their PCR amplifications and opine utilization profiles. A given chromosomal background harboured only one type of Ti plasmid within the strains from each analysed sample, showing a strong association between chromosomal backgrounds and Ti plasmids in A. vitis |
| format | article |
| id | ReDivia4282 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42822025-04-25T14:42:44Z Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours Palacio-Bielsa, Ana González-Abolafio, Raquel Álvarez, Belén Lastra, Beatriz Cambra, Miguel A. Salcedo, Carmina I. López, María M. Penyalver, Ramón Fifty-six tumorigenic Spanish grapevine strains of Agrobacterium spp. were tested for biovar classification, pathogenicity on several hosts, opine utilization, 16S rRNA gene sequencing and PCR amplifications using five primer sets targeting chromosomal and Ti plasmid genes. Fifty of them belonged to A. vitis (biovar 3), three to A. tumefaciens (biovar 1) and three to A. rhizogenes (biovar 2). All strains were tumorigenic on grapevines. Most A. vitis strains were also pathogenic on tomato and tobacco plants, while the three A. tumefaciens strains were only pathogenic on grapevine. Although most A. vitis strains used octopine, 12 utilized neither octopine nor nopaline. 16S rRNA gene sequencing clearly distinguished between strains belonging to the three species. Those of A. vitis could be further divided into three chromosomal backgrounds according to their 16S ribosomal RNA gene sequences. No universal primer pair was found for the detection of all three Agrobacterium species isolated from grapevine. DNA from all A. vitis strains was amplified with the chromosomally-encoded pehA primer pair. In both A. vitis and A. tumefaciens a correlation was observed between the amplifications obtained using the tmr and the virA Ti-plasmid-targeting primer pairs. Three types of Ti plasmid were found in A. vitis strains according to their PCR amplifications and opine utilization profiles. A given chromosomal background harboured only one type of Ti plasmid within the strains from each analysed sample, showing a strong association between chromosomal backgrounds and Ti plasmids in A. vitis 2017-06-01T10:09:46Z 2017-06-01T10:09:46Z 2009 JUN 2009 article Palacio-Bielsa, A., Gonzalez-Abolafio, R., Alvarez, B., Lastra, B., Cambra, M.A., Salcedo, C. I., Lopez, M.M., Penyalver, R. (2009). Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours. Plant Pathology, 58(3), 584-593. 0032-0862 http://hdl.handle.net/20.500.11939/4282 10.1111/j.1365-3059.2008.01984.x en openAccess Impreso |
| spellingShingle | Palacio-Bielsa, Ana González-Abolafio, Raquel Álvarez, Belén Lastra, Beatriz Cambra, Miguel A. Salcedo, Carmina I. López, María M. Penyalver, Ramón Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title | Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title_full | Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title_fullStr | Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title_full_unstemmed | Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title_short | Chromosomal and Ti plasmid characterization of tumorigenic strains of three Agrobacterium species isolated from grapevine tumours |
| title_sort | chromosomal and ti plasmid characterization of tumorigenic strains of three agrobacterium species isolated from grapevine tumours |
| url | http://hdl.handle.net/20.500.11939/4282 |
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