Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007)
PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid. Consequently, there is a shift in research towards DNA-based techniques. Although reports already exist on a...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4279 |
| _version_ | 1855032094160322560 |
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| author | Palacio-Bielsa, Ana Cambra, Miguel A. López, María M. |
| author_browse | Cambra, Miguel A. López, María M. Palacio-Bielsa, Ana |
| author_facet | Palacio-Bielsa, Ana Cambra, Miguel A. López, María M. |
| author_sort | Palacio-Bielsa, Ana |
| collection | ReDivia |
| description | PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid. Consequently, there is a shift in research towards DNA-based techniques. Although reports already exist on a variety of PCR-based fingerprinting assays used to analyse the genetic diversity of bacterial populations and define their relationships, this review focuses on the general use of PCR in phytobacteriology for detection and diagnosis purposes. An updated and detailed list of published PCR protocols for detection and identification of plant-pathogenic bacteria is presented and discussed, aimed at facilitating access to information that could be particularly useful for diagnostic laboratories. This compilation includes and discusses 246 articles published between 1989 and 2007 addressing 23 genera, more than 50 species, 10 subspecies and more than 40 pathovars. |
| format | article |
| id | ReDivia4279 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42792025-04-25T14:42:43Z Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) Palacio-Bielsa, Ana Cambra, Miguel A. López, María M. PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid. Consequently, there is a shift in research towards DNA-based techniques. Although reports already exist on a variety of PCR-based fingerprinting assays used to analyse the genetic diversity of bacterial populations and define their relationships, this review focuses on the general use of PCR in phytobacteriology for detection and diagnosis purposes. An updated and detailed list of published PCR protocols for detection and identification of plant-pathogenic bacteria is presented and discussed, aimed at facilitating access to information that could be particularly useful for diagnostic laboratories. This compilation includes and discusses 246 articles published between 1989 and 2007 addressing 23 genera, more than 50 species, 10 subspecies and more than 40 pathovars. 2017-06-01T10:09:46Z 2017-06-01T10:09:46Z 2009 JUL 2009 article Palacio-Bielsa, A., Cambra, M.A., Lopez, M.M. (2009). Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007). Journal of Plant Pathology, 91(2), 249-297. 1125-4653 http://hdl.handle.net/20.500.11939/4279 http://hdl.handle.net/20.500.11939/4279 10.4454/jpp.v91i2.957 en openAccess Impreso |
| spellingShingle | Palacio-Bielsa, Ana Cambra, Miguel A. López, María M. Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title | Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title_full | Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title_fullStr | Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title_full_unstemmed | Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title_short | Pcr Detection and Identification of Plant-Pathogenic Bacteria: Updated Review of Protocols (1989-2007) |
| title_sort | pcr detection and identification of plant pathogenic bacteria updated review of protocols 1989 2007 |
| url | http://hdl.handle.net/20.500.11939/4279 http://hdl.handle.net/20.500.11939/4279 |
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