HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism
We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4265 https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2012.05031.x |
| _version_ | 1855491722497228800 |
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| author | Oomen, Ronald J. F. J. Benito, Begona Sentenac, Herve Rodriguez-Navarro, Alonso Talón, Manuel Very, Anne-Alienor Domingo, Concha |
| author_browse | Benito, Begona Domingo, Concha Oomen, Ronald J. F. J. Rodriguez-Navarro, Alonso Sentenac, Herve Talón, Manuel Very, Anne-Alienor |
| author_facet | Oomen, Ronald J. F. J. Benito, Begona Sentenac, Herve Rodriguez-Navarro, Alonso Talón, Manuel Very, Anne-Alienor Domingo, Concha |
| author_sort | Oomen, Ronald J. F. J. |
| collection | ReDivia |
| description | We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5' region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3' region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions. |
| format | Artículo |
| id | ReDivia4265 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42652025-04-25T14:42:41Z HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism Oomen, Ronald J. F. J. Benito, Begona Sentenac, Herve Rodriguez-Navarro, Alonso Talón, Manuel Very, Anne-Alienor Domingo, Concha We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5' region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3' region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions. 2017-06-01T10:09:43Z 2017-06-01T10:09:43Z 2012 SEP 2012 article acceptedVersion Oomen, Ronald J. F. J., Benito, Begona, Sentenac, Herve, Rodriguez-Navarro, Alonso, Talón, M., Very, Anne-Alienor, Domingo, C. (2012). HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism. Plant Journal, 71(5), 750-762. 0960-7412 http://hdl.handle.net/20.500.11939/4265 10.1111/j.1365-313X.2012.05031.x https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2012.05031.x en openAccess Impreso |
| spellingShingle | Oomen, Ronald J. F. J. Benito, Begona Sentenac, Herve Rodriguez-Navarro, Alonso Talón, Manuel Very, Anne-Alienor Domingo, Concha HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title | HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title_full | HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title_fullStr | HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title_full_unstemmed | HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title_short | HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism |
| title_sort | hkt2 2 1 a k permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism |
| url | http://hdl.handle.net/20.500.11939/4265 https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2012.05031.x |
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