Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA

Two techniques for simultaneous detection and typing of plum pox potyvirus (PPV) isolates belonging to the D or M serotypes, heminested PCR (H-PCR) and PCR-ELISA, have been developed. Ten PPV isolates typed using PPV-D and PPV-M specific monoclonal antibodies by ELISA-DASI were used to validate thes...

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Bibliographic Details
Main Authors: Olmos, Antonio, Cambra, Mariano, Dasi, M. A., Candresse, Thierry, Esteban, Olga, Gorris, María T., Asensio, M.
Format: Artículo
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4259

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