Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses
A new and highly sensitive method for the amplification of viral RNA targets from plant material has been developed and patented. This technique called Co-operational amplification (Co-PCR) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. T...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4255 |
| _version_ | 1855032090144276480 |
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| author | Olmos, Antonio Bertolini, Edson Cambra, Mariano |
| author_browse | Bertolini, Edson Cambra, Mariano Olmos, Antonio |
| author_facet | Olmos, Antonio Bertolini, Edson Cambra, Mariano |
| author_sort | Olmos, Antonio |
| collection | ReDivia |
| description | A new and highly sensitive method for the amplification of viral RNA targets from plant material has been developed and patented. This technique called Co-operational amplification (Co-PCR) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. The reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination of the two pair of primers, one pair external to other; and the co-operational action of amplicons for the production of the largest fragment. The technique was used successfully, both in metal block and capillary air thermal cyclers for the detection of plant RNA viruses (Cherry leaf roll virus, Strawberry latent ringspot virus, Cucumber mosaic virus, Plum pox virus and Citrus tristeza virus). The sensitivity observed is at least 100 times higher than that achieved with RT-PCR and similar to nested RT-PCR. Colorimetric detection was coupled with this methodology facilitating its introduction for routine indexing programs and for phytosanitary selection of virus-free plant material. (C) 2002 Elsevier Science B.V. All rights reserved. |
| format | article |
| id | ReDivia4255 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42552025-04-25T14:42:40Z Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses Olmos, Antonio Bertolini, Edson Cambra, Mariano A new and highly sensitive method for the amplification of viral RNA targets from plant material has been developed and patented. This technique called Co-operational amplification (Co-PCR) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. The reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination of the two pair of primers, one pair external to other; and the co-operational action of amplicons for the production of the largest fragment. The technique was used successfully, both in metal block and capillary air thermal cyclers for the detection of plant RNA viruses (Cherry leaf roll virus, Strawberry latent ringspot virus, Cucumber mosaic virus, Plum pox virus and Citrus tristeza virus). The sensitivity observed is at least 100 times higher than that achieved with RT-PCR and similar to nested RT-PCR. Colorimetric detection was coupled with this methodology facilitating its introduction for routine indexing programs and for phytosanitary selection of virus-free plant material. (C) 2002 Elsevier Science B.V. All rights reserved. 2017-06-01T10:09:41Z 2017-06-01T10:09:41Z 2002 OCT 2002 article Olmos, A., Bertolini, E., Cambra, M. (2002). Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses. Journal of virological methods, 106(1), 51-59. 0166-0934 http://hdl.handle.net/20.500.11939/4255 10.1016/S0166-0934(02)00132-5 en openAccess Impreso |
| spellingShingle | Olmos, Antonio Bertolini, Edson Cambra, Mariano Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title | Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title_full | Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title_fullStr | Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title_full_unstemmed | Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title_short | Simultaneous and co-operational amplification (Co-PCR): a new concept for detection of plant viruses |
| title_sort | simultaneous and co operational amplification co pcr a new concept for detection of plant viruses |
| url | http://hdl.handle.net/20.500.11939/4255 |
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