Isothermal amplification for detection of Plum pox virus
A nucleic acid sequence-based amplification method coupled with flow-through hybridisation (NASBA-FH) was developed for Plum pox virus (PPV) detection. The detection limit of the NASBA-FH was established at two copies of control transcripts, resulting 10 times higher than that obtained by Co-PCR and...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Objeto de conferencia |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4254 |
| _version_ | 1855491720852013056 |
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| author | Olmos, Antonio Bertolini, Edson Cambra, Mariano |
| author2 | Ertunc, F. |
| author_browse | Bertolini, Edson Cambra, Mariano Ertunc, F. Olmos, Antonio |
| author_facet | Ertunc, F. Olmos, Antonio Bertolini, Edson Cambra, Mariano |
| author_sort | Olmos, Antonio |
| collection | ReDivia |
| description | A nucleic acid sequence-based amplification method coupled with flow-through hybridisation (NASBA-FH) was developed for Plum pox virus (PPV) detection. The detection limit of the NASBA-FH was established at two copies of control transcripts, resulting 10 times higher than that obtained by Co-PCR and 1000 times higher than the sensitivity afforded by RT-PCR. In addition, samples from 253 stone-fruit trees were collected during winter and analysed. The samples were tested using methods recommended by the European and Mediterranean Plant Protection Organization to detect PPV (DASI-ELISA, RT-PCR and Co-PCR) and by NASBA-FH. PPV diagnosis by ELISA and NASBA-FH coincided in 90.5%, while ELISA and PCR-based methods coincided in the diagnosis of 91.3% of the trees and PCR-based methods with NASBA-FH agreed in 95.2%. Results support that NASBA-FH is a suitable molecular method for routine PPV detection in the winter period. |
| format | Objeto de conferencia |
| id | ReDivia4254 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42542025-04-25T14:51:35Z Isothermal amplification for detection of Plum pox virus ACTA HORTICULTURAE Olmos, Antonio Bertolini, Edson Cambra, Mariano Ertunc, F. Caglayan, Kadriye A nucleic acid sequence-based amplification method coupled with flow-through hybridisation (NASBA-FH) was developed for Plum pox virus (PPV) detection. The detection limit of the NASBA-FH was established at two copies of control transcripts, resulting 10 times higher than that obtained by Co-PCR and 1000 times higher than the sensitivity afforded by RT-PCR. In addition, samples from 253 stone-fruit trees were collected during winter and analysed. The samples were tested using methods recommended by the European and Mediterranean Plant Protection Organization to detect PPV (DASI-ELISA, RT-PCR and Co-PCR) and by NASBA-FH. PPV diagnosis by ELISA and NASBA-FH coincided in 90.5%, while ELISA and PCR-based methods coincided in the diagnosis of 91.3% of the trees and PCR-based methods with NASBA-FH agreed in 95.2%. Results support that NASBA-FH is a suitable molecular method for routine PPV detection in the winter period. 2017-06-01T10:09:41Z 2017-06-01T10:09:41Z 2008 2008 conferenceObject Olmos, A., Bertolini, E., Cambra, M. (2008). Isothermal amplification for detection of Plum pox virus. Proceedings of the Twentieth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops - Fruit Tree Diseases, (781), 209-213. 0567-7572; 978-90-6605-080-8 http://hdl.handle.net/20.500.11939/4254 10.17660/ActaHortic.2008.781.31 en openAccess Impreso |
| spellingShingle | Olmos, Antonio Bertolini, Edson Cambra, Mariano Isothermal amplification for detection of Plum pox virus |
| title | Isothermal amplification for detection of Plum pox virus |
| title_full | Isothermal amplification for detection of Plum pox virus |
| title_fullStr | Isothermal amplification for detection of Plum pox virus |
| title_full_unstemmed | Isothermal amplification for detection of Plum pox virus |
| title_short | Isothermal amplification for detection of Plum pox virus |
| title_sort | isothermal amplification for detection of plum pox virus |
| url | http://hdl.handle.net/20.500.11939/4254 |
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