New device and method for capture, reverse transcription and nested PCR in a single closed-tube
A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4252 |
| _version_ | 1855032089801392128 |
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| author | Olmos, Antonio Cambra, Mariano Esteban, Olga Gorris, María T. Terrada, E. |
| author_browse | Cambra, Mariano Esteban, Olga Gorris, María T. Olmos, Antonio Terrada, E. |
| author_facet | Olmos, Antonio Cambra, Mariano Esteban, Olga Gorris, María T. Terrada, E. |
| author_sort | Olmos, Antonio |
| collection | ReDivia |
| description | A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-PCR. The method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum pox potyvirus isolates in plant tissues and single aphids squashed on paper. This device and methodology could be easily adapted to the detection of other targets. |
| format | article |
| id | ReDivia4252 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42522025-04-25T14:42:39Z New device and method for capture, reverse transcription and nested PCR in a single closed-tube Olmos, Antonio Cambra, Mariano Esteban, Olga Gorris, María T. Terrada, E. A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-PCR. The method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum pox potyvirus isolates in plant tissues and single aphids squashed on paper. This device and methodology could be easily adapted to the detection of other targets. 2017-06-01T10:09:41Z 2017-06-01T10:09:41Z 1999 MAR 15 1999 article publishedVersion Olmos, A., Cambra, M., Esteban, O., Gorris, M. T., Terrada, E. (1999). New device and method for capture, reverse transcription and nested PCR in a single closed-tube. Nucleic acids research, 27(6), 1564-1565. 0305-1048 http://hdl.handle.net/20.500.11939/4252 10.1093/nar/27.6.1564 en openAccess Impreso |
| spellingShingle | Olmos, Antonio Cambra, Mariano Esteban, Olga Gorris, María T. Terrada, E. New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title | New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title_full | New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title_fullStr | New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title_full_unstemmed | New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title_short | New device and method for capture, reverse transcription and nested PCR in a single closed-tube |
| title_sort | new device and method for capture reverse transcription and nested pcr in a single closed tube |
| url | http://hdl.handle.net/20.500.11939/4252 |
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