Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome
The availability of a saturated genetic map of Clementine was identified by the International Citrus Genome Consortium as an essential prerequisite to assist the assembly of the reference whole genome sequence based on a Clementine derived haploid. The primary goals of the present study were to esta...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Otros Autores: | |
| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4244 https://doi.org/10.17660/ActaHortic.2015.1065.71 |
| _version_ | 1855491719724793856 |
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| author | Ollitrault, Patrick Terol, Javier Chen, Chunxian Federici, Claire T. Lotfy, Samia Hippolyte, Isabelle Ollitrault, Frederique Berard, Aurelie Chauveau, Aurelie Cuenca, José Costantino, Gilles Kacar, Yildiz Aka Mu, Lisa García-Lor, Andrés Curk, Franck Froelicher, Yann Aleza, Pablo Boland, Anne Billot, Claire Navarro, Luis Luro, Francois Roose, Mikeal L. Gmitter Jr., Frederick G. Talón, Manuel Brunel, Dominique |
| author2 | Sabater-Munoz, Beatriz |
| author_browse | Aleza, Pablo Berard, Aurelie Billot, Claire Boland, Anne Brunel, Dominique Chauveau, Aurelie Chen, Chunxian Costantino, Gilles Cuenca, José Curk, Franck Federici, Claire T. Froelicher, Yann García-Lor, Andrés Gmitter Jr., Frederick G. Hippolyte, Isabelle Kacar, Yildiz Aka Lotfy, Samia Luro, Francois Mu, Lisa Navarro, Luis Ollitrault, Frederique Ollitrault, Patrick Roose, Mikeal L. Sabater-Munoz, Beatriz Talón, Manuel Terol, Javier |
| author_facet | Sabater-Munoz, Beatriz Ollitrault, Patrick Terol, Javier Chen, Chunxian Federici, Claire T. Lotfy, Samia Hippolyte, Isabelle Ollitrault, Frederique Berard, Aurelie Chauveau, Aurelie Cuenca, José Costantino, Gilles Kacar, Yildiz Aka Mu, Lisa García-Lor, Andrés Curk, Franck Froelicher, Yann Aleza, Pablo Boland, Anne Billot, Claire Navarro, Luis Luro, Francois Roose, Mikeal L. Gmitter Jr., Frederick G. Talón, Manuel Brunel, Dominique |
| author_sort | Ollitrault, Patrick |
| collection | ReDivia |
| description | The availability of a saturated genetic map of Clementine was identified by the International Citrus Genome Consortium as an essential prerequisite to assist the assembly of the reference whole genome sequence based on a Clementine derived haploid. The primary goals of the present study were to establish a Clementine reference map, and to perform comparative mapping with pummelo and sweet orange. Five parental genetic maps were established with SNPs, SSRs and InDels. A medium density reference map (961 markers for 1084.1 cM) of Clementine was established and used by the ICGC to facilitate the chromosome assembly of the haploid genome sequence. Comparative mapping with pummelo and sweet orange revealed that the linear order of markers was highly conserved. Reasonable inferences of most citrus genomes should be obtained by mapping next-generation sequencing data against the haploid reference genome sequence. Skewed segregations were frequent and higher in the male than female Clementine potentially leading to false interpretation of the genetic determinism of phenotypic traits. The mapping data confirmed that Clementine arose from hybridization between 'Mediterranean' mandarin and sweet orange and identified nine recombination break points for the sweet orange gamete that contributed to the Clementine genome. Introgression of pummelo genome fragments were identified in heterozygosity in each chromosome. Moreover, it appeared that the genome of the haploid Clementine used to establish the citrus reference genome sequence was inherited primarily from the 'Mediterranean' mandarin. The usefulness of this genetic map, anchored in the reference whole genome sequence, is discussed. |
| format | Objeto de conferencia |
| id | ReDivia4244 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42442025-04-25T14:51:36Z Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome Acta Horticulturae Ollitrault, Patrick Terol, Javier Chen, Chunxian Federici, Claire T. Lotfy, Samia Hippolyte, Isabelle Ollitrault, Frederique Berard, Aurelie Chauveau, Aurelie Cuenca, José Costantino, Gilles Kacar, Yildiz Aka Mu, Lisa García-Lor, Andrés Curk, Franck Froelicher, Yann Aleza, Pablo Boland, Anne Billot, Claire Navarro, Luis Luro, Francois Roose, Mikeal L. Gmitter Jr., Frederick G. Talón, Manuel Brunel, Dominique Sabater-Munoz, Beatriz The availability of a saturated genetic map of Clementine was identified by the International Citrus Genome Consortium as an essential prerequisite to assist the assembly of the reference whole genome sequence based on a Clementine derived haploid. The primary goals of the present study were to establish a Clementine reference map, and to perform comparative mapping with pummelo and sweet orange. Five parental genetic maps were established with SNPs, SSRs and InDels. A medium density reference map (961 markers for 1084.1 cM) of Clementine was established and used by the ICGC to facilitate the chromosome assembly of the haploid genome sequence. Comparative mapping with pummelo and sweet orange revealed that the linear order of markers was highly conserved. Reasonable inferences of most citrus genomes should be obtained by mapping next-generation sequencing data against the haploid reference genome sequence. Skewed segregations were frequent and higher in the male than female Clementine potentially leading to false interpretation of the genetic determinism of phenotypic traits. The mapping data confirmed that Clementine arose from hybridization between 'Mediterranean' mandarin and sweet orange and identified nine recombination break points for the sweet orange gamete that contributed to the Clementine genome. Introgression of pummelo genome fragments were identified in heterozygosity in each chromosome. Moreover, it appeared that the genome of the haploid Clementine used to establish the citrus reference genome sequence was inherited primarily from the 'Mediterranean' mandarin. The usefulness of this genetic map, anchored in the reference whole genome sequence, is discussed. 2017-06-01T10:09:40Z 2017-06-01T10:09:40Z 2015 2015 conferenceObject Patrick Ollitrault, Javier Terol, Chunxian Chen, Claire T. Federici, Samia Lotfy, Isabelle Hippolyte, Frédérique Ollitrault, Aurélie Bérard, Aurélie Chauveau, José Cuenca, Gilles Costantino, Yildiz Aka Kacar, Lisa Mu, Andres Garcia-Lor, Franck Curk, Yann Froelicher, Pablo Aleza, Anne Boland, Claire Billot, Luis Navarro, François Luro, Mikeal L. Roose, Frederick G. Gmitter Jr., Manuel Talón and Dominique Brunel (2015). Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome. Acta Hortic. 1065, 561-573 0567-7572; 978-94-62610-53-8 http://hdl.handle.net/20.500.11939/4244 https://doi.org/10.17660/ActaHortic.2015.1065.71 en openAccess Impreso |
| spellingShingle | Ollitrault, Patrick Terol, Javier Chen, Chunxian Federici, Claire T. Lotfy, Samia Hippolyte, Isabelle Ollitrault, Frederique Berard, Aurelie Chauveau, Aurelie Cuenca, José Costantino, Gilles Kacar, Yildiz Aka Mu, Lisa García-Lor, Andrés Curk, Franck Froelicher, Yann Aleza, Pablo Boland, Anne Billot, Claire Navarro, Luis Luro, Francois Roose, Mikeal L. Gmitter Jr., Frederick G. Talón, Manuel Brunel, Dominique Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title | Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title_full | Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title_fullStr | Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title_full_unstemmed | Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title_short | Comparative Genetic Mapping between Clementine, Pummelo and Sweet Orange and the Interspecicic Structure of the Clementine Genome |
| title_sort | comparative genetic mapping between clementine pummelo and sweet orange and the interspecicic structure of the clementine genome |
| url | http://hdl.handle.net/20.500.11939/4244 https://doi.org/10.17660/ActaHortic.2015.1065.71 |
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