Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus
Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapp...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4242 |
| _version_ | 1855491719355695104 |
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| author | Ollitrault, Frederique Terol, Javier Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick |
| author_browse | Navarro, Luis Ollitrault, Frederique Ollitrault, Patrick Pina, José A. Talón, Manuel Terol, Javier |
| author_facet | Ollitrault, Frederique Terol, Javier Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick |
| author_sort | Ollitrault, Frederique |
| collection | ReDivia |
| description | Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Methods and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. Conclusions: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps. |
| format | Artículo |
| id | ReDivia4242 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42422025-04-25T14:42:38Z Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus Ollitrault, Frederique Terol, Javier Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick Premise of the study: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. Methods and Results: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. Conclusions: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps. 2017-06-01T10:09:40Z 2017-06-01T10:09:40Z 2010 NOV 2010 article Ollitrault, Frederique, Terol, J., Pina, J.A., Navarro, L., Talón, M., Ollitrault, P. (2010). Development of Ssr Markers from Citrus Clementina (Rutaceae), Bac End Sequences and Interspecific Transferability in Citrus. American Journal of Botany, 97(11), E124-E129. 0002-9122 http://hdl.handle.net/20.500.11939/4242 10.3732/ajb.1000280 en openAccess Impreso |
| spellingShingle | Ollitrault, Frederique Terol, Javier Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title | Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title_full | Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title_fullStr | Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title_full_unstemmed | Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title_short | Development of Ssr Markers from Citrus Clementina (Rutaceae) Bac End Sequences and Interspecific Transferability in Citrus |
| title_sort | development of ssr markers from citrus clementina rutaceae bac end sequences and interspecific transferability in citrus |
| url | http://hdl.handle.net/20.500.11939/4242 |
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