Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus
Premise of the study: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. Methods and Results: Using po...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4241 |
| _version_ | 1855491719183728640 |
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| author | Ollitrault, Frederique Terol, Javier Martin, Alena Alonso Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick |
| author_browse | Martin, Alena Alonso Navarro, Luis Ollitrault, Frederique Ollitrault, Patrick Pina, José A. Talón, Manuel Terol, Javier |
| author_facet | Ollitrault, Frederique Terol, Javier Martin, Alena Alonso Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick |
| author_sort | Ollitrault, Frederique |
| collection | ReDivia |
| description | Premise of the study: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. Methods and Results: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed. Conclusions: These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments. |
| format | Artículo |
| id | ReDivia4241 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42412025-04-25T14:42:38Z Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus Ollitrault, Frederique Terol, Javier Martin, Alena Alonso Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick Premise of the study: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. Methods and Results: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed. Conclusions: These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments. 2017-06-01T10:09:40Z 2017-06-01T10:09:40Z 2012 JUL 2012 article Ollitrault, Frederique, Terol, J., Martin, Alena Alonso, Pina, J.A., Navarro, L., Talón, M., Ollitrault, P. (2012). Development of Indel Markers from Citrus Clementina (Rutaceae), Bac-End Sequences and Interspecific Transferability in Citrus. American Journal of Botany, 99(7), E268-E273. 0002-9122 http://hdl.handle.net/20.500.11939/4241 10.3732/ajb.1100569 en openAccess Impreso |
| spellingShingle | Ollitrault, Frederique Terol, Javier Martin, Alena Alonso Pina, José A. Navarro, Luis Talón, Manuel Ollitrault, Patrick Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title | Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title_full | Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title_fullStr | Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title_full_unstemmed | Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title_short | Development of Indel Markers from Citrus Clementina (Rutaceae) Bac-End Sequences and Interspecific Transferability in Citrus |
| title_sort | development of indel markers from citrus clementina rutaceae bac end sequences and interspecific transferability in citrus |
| url | http://hdl.handle.net/20.500.11939/4241 |
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