Green fluorescent protein as a visual marker in somatic hybridization
Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression...
| Main Authors: | , , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4229 |
| _version_ | 1855032086595895296 |
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| author | Olivares-Fuster, Oscar Pena, Leandro Durán-Vila, Núria Navarro, Luis |
| author_browse | Durán-Vila, Núria Navarro, Luis Olivares-Fuster, Oscar Pena, Leandro |
| author_facet | Olivares-Fuster, Oscar Pena, Leandro Durán-Vila, Núria Navarro, Luis |
| author_sort | Olivares-Fuster, Oscar |
| collection | ReDivia |
| description | Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants. |
| format | article |
| id | ReDivia4229 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42292025-04-25T14:42:36Z Green fluorescent protein as a visual marker in somatic hybridization Olivares-Fuster, Oscar Pena, Leandro Durán-Vila, Núria Navarro, Luis Using a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants. 2017-06-01T10:09:38Z 2017-06-01T10:09:38Z 2002 APR article Olivares-Fuster, O., Pena, L., Duran-Vila, N., Navarro, L. (2002). Green fluorescent protein as a visual marker in somatic hybridization. Annals of Botany, 89(4), 491-497. 0305-7364 http://hdl.handle.net/20.500.11939/4229 10.1093/aob/mcf054 en openAccess Impreso |
| spellingShingle | Olivares-Fuster, Oscar Pena, Leandro Durán-Vila, Núria Navarro, Luis Green fluorescent protein as a visual marker in somatic hybridization |
| title | Green fluorescent protein as a visual marker in somatic hybridization |
| title_full | Green fluorescent protein as a visual marker in somatic hybridization |
| title_fullStr | Green fluorescent protein as a visual marker in somatic hybridization |
| title_full_unstemmed | Green fluorescent protein as a visual marker in somatic hybridization |
| title_short | Green fluorescent protein as a visual marker in somatic hybridization |
| title_sort | green fluorescent protein as a visual marker in somatic hybridization |
| url | http://hdl.handle.net/20.500.11939/4229 |
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