Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread
Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral rep...
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4218 |
| _version_ | 1855032084929708032 |
|---|---|
| author | Niehl, Annette Pasquier, Adrien Ferriol, Inmaculada Mely, Yves Heinlein, Manfred |
| author_browse | Ferriol, Inmaculada Heinlein, Manfred Mely, Yves Niehl, Annette Pasquier, Adrien |
| author_facet | Niehl, Annette Pasquier, Adrien Ferriol, Inmaculada Mely, Yves Heinlein, Manfred |
| author_sort | Niehl, Annette |
| collection | ReDivia |
| description | Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MPTMV. We show that MPORMV supports TMV movement more efficiently than MPTMV. Moreover, MPORMV localizes to C-MERs like MPTMV but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MPTMV. Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement. (C) 2014 Elsevier Inc. All rights reserved. |
| format | article |
| id | ReDivia4218 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42182025-04-25T14:42:34Z Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread Niehl, Annette Pasquier, Adrien Ferriol, Inmaculada Mely, Yves Heinlein, Manfred Tobacco mosaic virus (TMV) is a longstanding model for studying virus movement and macromolecular transport through plasmodesmata (PD). Its movement protein (MP) interacts with cortical microtubule (MT)-associated ER sites (C-MERs) to facilitate the formation and transport of ER-associated viral replication complexes (VRCs) along the ER-actin network towards PD. To investigate whether this movement mechanism might be conserved between tobamoviruses, we compared the functions of Oilseed rape mosaic virus (ORMV) MP with those of MPTMV. We show that MPORMV supports TMV movement more efficiently than MPTMV. Moreover, MPORMV localizes to C-MERs like MPTMV but accumulates to lower levels and does not localize to larger inclusions/VRCs or along MTs, patterns regularly seen for MPTMV. Our findings extend the role of C-MERs in viral cell-to-cell transport to a virus commonly used for functional genomics in Arabidopsis. Moreover, accumulation of tobamoviral MP in inclusions or along MTs is not required for virus movement. (C) 2014 Elsevier Inc. All rights reserved. 2017-06-01T10:09:37Z 2017-06-01T10:09:37Z 2014 MAY 2014 article Niehl, Annette, Pasquier, Adrien, Ferriol, I., Mely, Yves, Heinlein, Manfred (2014). Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP), reveals common and dissimilar MP functions for tobamovirus spread. Virology, 456, 43-54. 0042-6822 http://hdl.handle.net/20.500.11939/4218 10.1016/j.virol.2014.03.007 en openAccess Impreso |
| spellingShingle | Niehl, Annette Pasquier, Adrien Ferriol, Inmaculada Mely, Yves Heinlein, Manfred Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title | Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title_full | Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title_fullStr | Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title_full_unstemmed | Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title_short | Comparison of the Oilseed rape mosaic virus and Tobacco mosaic virus movement proteins (MP) reveals common and dissimilar MP functions for tobamovirus spread |
| title_sort | comparison of the oilseed rape mosaic virus and tobacco mosaic virus movement proteins mp reveals common and dissimilar mp functions for tobamovirus spread |
| url | http://hdl.handle.net/20.500.11939/4218 |
| work_keys_str_mv | AT niehlannette comparisonoftheoilseedrapemosaicvirusandtobaccomosaicvirusmovementproteinsmprevealscommonanddissimilarmpfunctionsfortobamovirusspread AT pasquieradrien comparisonoftheoilseedrapemosaicvirusandtobaccomosaicvirusmovementproteinsmprevealscommonanddissimilarmpfunctionsfortobamovirusspread AT ferriolinmaculada comparisonoftheoilseedrapemosaicvirusandtobaccomosaicvirusmovementproteinsmprevealscommonanddissimilarmpfunctionsfortobamovirusspread AT melyyves comparisonoftheoilseedrapemosaicvirusandtobaccomosaicvirusmovementproteinsmprevealscommonanddissimilarmpfunctionsfortobamovirusspread AT heinleinmanfred comparisonoftheoilseedrapemosaicvirusandtobaccomosaicvirusmovementproteinsmprevealscommonanddissimilarmpfunctionsfortobamovirusspread |