Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains
Properties of the unusual sour cherry strain of plum pox potyvirus (PPV-SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4216 |
| _version_ | 1855491715231645696 |
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| author | Nemchinov, L. Hadidi, Ahmed Maiss, E. Cambra, Mariano Candresse, Thierry Damsteegt, Vern |
| author_browse | Cambra, Mariano Candresse, Thierry Damsteegt, Vern Hadidi, Ahmed Maiss, E. Nemchinov, L. |
| author_facet | Nemchinov, L. Hadidi, Ahmed Maiss, E. Cambra, Mariano Candresse, Thierry Damsteegt, Vern |
| author_sort | Nemchinov, L. |
| collection | ReDivia |
| description | Properties of the unusual sour cherry strain of plum pox potyvirus (PPV-SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV-SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV-cherry (PPV-C), and that PPV-SoC is its prototype member. |
| format | Artículo |
| id | ReDivia4216 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42162025-04-25T14:42:34Z Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains Nemchinov, L. Hadidi, Ahmed Maiss, E. Cambra, Mariano Candresse, Thierry Damsteegt, Vern Properties of the unusual sour cherry strain of plum pox potyvirus (PPV-SoC) were investigated by sequencing its 3'-terminal 1,360 nt and examining its serological reactivity with several monoclonal antibodies (MAbs) to PPV. A significantly low degree of identity of the sequenced region has been found between PPV-SoC and other isolates of PPV. This is due to the high nucleotide divergence between the 5'-terminal region of the coat protein (CP) coding region of PPV-SoC and that of other isolates. Most nucleotide substitutions in the sequenced region are not silent and result in amino acid changes, especially near the N terminus of the CP. The unique 5' terminus of the PPV-SoC CP coding sequence has been utilized to develop a cRNA probe that hybridizes to PPV-SoC but not to other isolates/members of the D or M subgroups of PPV. The N-terminal region of the PPV-SoC CP contains the recognizable DAG motif that determines aphid transmissibility of potyviruses. Aphid transmission of PPV-SoC to herbaceous and woody hosts has been confirmed experimentally. Serological reactivity of PPV-SoC with 10 MAbs of PPV suggest that PPV-SoC represents a new serotype of PPV that does not fall into the conventional D or M serotypes/subgroups. We propose that PPV-SoC represents a new subgroup of PPV strains, termed PPV-cherry (PPV-C), and that PPV-SoC is its prototype member. 2017-06-01T10:09:37Z 2017-06-01T10:09:37Z 1996 NOV 1996 article Nemchinov, L., Hadidi, A., Maiss, E., Cambra, M., Candresse, T., Damsteegt, V. (1996). Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains. Phytopathology, 86(11), 1215-1221. 0031-949X http://hdl.handle.net/20.500.11939/4216 10.1094/Phyto-86-1215 en openAccess Impreso |
| spellingShingle | Nemchinov, L. Hadidi, Ahmed Maiss, E. Cambra, Mariano Candresse, Thierry Damsteegt, Vern Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title | Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title_full | Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title_fullStr | Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title_full_unstemmed | Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title_short | Sour cherry strain of plum pox potyvirus (PPV): Molecular and serological evidence for a new subgroup of PPV strains |
| title_sort | sour cherry strain of plum pox potyvirus ppv molecular and serological evidence for a new subgroup of ppv strains |
| url | http://hdl.handle.net/20.500.11939/4216 |
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