Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool

Somaclonal variation is considered to be a useful source of variation in several fruit species. A study was developed for in vitro propagation and plant regeneration using callus from the primordial and young leaves of the persimmon cultivar ‘Rojo Brillante’, to study in subsequent experiments somac...

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Autores principales: Naval, María M., Llácer, Gerardo, Badenes, María L., Giordani, Edgardo
Otros Autores: Espiau, MT Alonso, JM
Formato: conferenceObject
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/4203
https://doi.org/10.17660/ActaHortic.2009.814.44
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author Naval, María M.
Llácer, Gerardo
Badenes, María L.
Giordani, Edgardo
author2 Espiau, MT Alonso, JM
author_browse Badenes, María L.
Espiau, MT Alonso, JM
Giordani, Edgardo
Llácer, Gerardo
Naval, María M.
author_facet Espiau, MT Alonso, JM
Naval, María M.
Llácer, Gerardo
Badenes, María L.
Giordani, Edgardo
author_sort Naval, María M.
collection ReDivia
description Somaclonal variation is considered to be a useful source of variation in several fruit species. A study was developed for in vitro propagation and plant regeneration using callus from the primordial and young leaves of the persimmon cultivar ‘Rojo Brillante’, to study in subsequent experiments somaclonal variation for qualitative and quantitative characters. The replacement of FeNaEDTA by FeEDDHA as iron source resulted in shoots of higher quality. Leaf explants taken from in vitro shoots were cultured on a modified Murashige and Skoog medium (MS½N) supplemented with various combinations of cytokinin (Z or BA) with different auxins (IAA or NAA). Optimal shoot regeneration was obtained with 9 mg L-1 (42 μM) zeatin and 0.1 mg L-1 (0.5 μM) NAA. Under these conditions, more than 20% of organogenic callus induction was obtained.
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spelling ReDivia42032025-04-25T14:53:14Z Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool Acta Horticulturae Naval, María M. Llácer, Gerardo Badenes, María L. Giordani, Edgardo Espiau, MT Alonso, JM Somaclonal variation is considered to be a useful source of variation in several fruit species. A study was developed for in vitro propagation and plant regeneration using callus from the primordial and young leaves of the persimmon cultivar ‘Rojo Brillante’, to study in subsequent experiments somaclonal variation for qualitative and quantitative characters. The replacement of FeNaEDTA by FeEDDHA as iron source resulted in shoots of higher quality. Leaf explants taken from in vitro shoots were cultured on a modified Murashige and Skoog medium (MS½N) supplemented with various combinations of cytokinin (Z or BA) with different auxins (IAA or NAA). Optimal shoot regeneration was obtained with 9 mg L-1 (42 μM) zeatin and 0.1 mg L-1 (0.5 μM) NAA. Under these conditions, more than 20% of organogenic callus induction was obtained. 2017-06-01T10:09:35Z 2017-06-01T10:09:35Z 2009 2009 conferenceObject Naval, M. M., Llacer, G., Badenes, M. L. & Giordani, E. (2009). Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.), cv. 'Rojo Brillante' as a Breeding Tool. Acta Horticulturae, 814, 291-294. 0567-7572; 978-90-6605-290-1 http://hdl.handle.net/20.500.11939/4203 https://doi.org/10.17660/ActaHortic.2009.814.44 en openAccess Impreso
spellingShingle Naval, María M.
Llácer, Gerardo
Badenes, María L.
Giordani, Edgardo
Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title_full Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title_fullStr Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title_full_unstemmed Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title_short Studies on the Somaclonal Variation of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' as a Breeding Tool
title_sort studies on the somaclonal variation of the persimmon diospyros kaki thunb cv rojo brillante as a breeding tool
url http://hdl.handle.net/20.500.11939/4203
https://doi.org/10.17660/ActaHortic.2009.814.44
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