Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante'
Conventional breeding of fruit trees is a slow process due to their long generation time as well as high degree of heterozygosis of these species. Biotechnology techniques and in vitro tissue culture, which includes genetic transformation, are considered promising for the improvement of these specie...
| Autores principales: | , , , |
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| Otros Autores: | |
| Formato: | conferenceObject |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4202 https://doi.org/10.17660/ActaHortic.2009.833.29 |
| _version_ | 1855032082235916288 |
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| author | Naval, María M. Llácer, Gerardo Badenes, María L. Giordani, Edgardo |
| author2 | Bellini, E. |
| author_browse | Badenes, María L. Bellini, E. Giordani, Edgardo Llácer, Gerardo Naval, María M. |
| author_facet | Bellini, E. Naval, María M. Llácer, Gerardo Badenes, María L. Giordani, Edgardo |
| author_sort | Naval, María M. |
| collection | ReDivia |
| description | Conventional breeding of fruit trees is a slow process due to their long generation time as well as high degree of heterozygosis of these species. Biotechnology techniques and in vitro tissue culture, which includes genetic transformation, are considered promising for the improvement of these species. An efficient protocol for regeneration of whole plants is a prerequisite for the development of an efficient Agrobacterium-mediated transformation system. The objective of this paper was to develop a protocol for plant regeneration of Diospyros kaki Thunb. cv. ‘Rojo Brillante’ via organogenesis from leaf explants. Combined phytohormones and dosage were evaluated for regeneration. The best regeneration efficiencies (29.2%) were obtained with 9 mg/L zeatin in combination with 0.1 mg/L naphtalenacetic acid. The cultures were maintained in the dark for 20 days followed by incubation under photoperiod conditions. Shoots were micrografted in vitro onto seedling rootstocks. The rootstocks came from Diospyros virginiana L. seedlings; they were micrografted after 6 to 8 days after germination. |
| format | conferenceObject |
| id | ReDivia4202 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia42022025-04-25T14:53:14Z Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' Acta Horticulturae Naval, María M. Llácer, Gerardo Badenes, María L. Giordani, Edgardo Bellini, E. Conventional breeding of fruit trees is a slow process due to their long generation time as well as high degree of heterozygosis of these species. Biotechnology techniques and in vitro tissue culture, which includes genetic transformation, are considered promising for the improvement of these species. An efficient protocol for regeneration of whole plants is a prerequisite for the development of an efficient Agrobacterium-mediated transformation system. The objective of this paper was to develop a protocol for plant regeneration of Diospyros kaki Thunb. cv. ‘Rojo Brillante’ via organogenesis from leaf explants. Combined phytohormones and dosage were evaluated for regeneration. The best regeneration efficiencies (29.2%) were obtained with 9 mg/L zeatin in combination with 0.1 mg/L naphtalenacetic acid. The cultures were maintained in the dark for 20 days followed by incubation under photoperiod conditions. Shoots were micrografted in vitro onto seedling rootstocks. The rootstocks came from Diospyros virginiana L. seedlings; they were micrografted after 6 to 8 days after germination. 2017-06-01T10:09:34Z 2017-06-01T10:09:34Z 2009 2009 conferenceObject Naval, M. M., Llacer, G., Badenes, M. L. & Giordani, E. (2009). Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.), cv. 'Rojo Brillante'. Acta Horticulturae, 833, 183-186. 0567-7572; 978-90-6605-542-1 http://hdl.handle.net/20.500.11939/4202 https://doi.org/10.17660/ActaHortic.2009.833.29 en openAccess Impreso |
| spellingShingle | Naval, María M. Llácer, Gerardo Badenes, María L. Giordani, Edgardo Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title | Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title_full | Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title_fullStr | Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title_full_unstemmed | Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title_short | Adventitious Shoot Regeneration from Leaf Explants of the Persimmon (Diospyros kaki Thunb.) cv. 'Rojo Brillante' |
| title_sort | adventitious shoot regeneration from leaf explants of the persimmon diospyros kaki thunb cv rojo brillante |
| url | http://hdl.handle.net/20.500.11939/4202 https://doi.org/10.17660/ActaHortic.2009.833.29 |
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