Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs
The “Maladie des feuilles cassantes” (MFC) or “Brittle leaf disease” of date palms is associated with the accumulation of two populations of small, chloroplast-encoded RNAs. A plasmid vector containing a cDNA with partial sequences of both of these RNA populations was used to synthesize a DIG-labele...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4199 |
| _version_ | 1855491712257884160 |
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| author | Namsi, A. Marques, Jorge Fadda, Z. Takrouni, M. L. Ben Mahamoud, O. Zouba, Anis Daros, José A. Flores, R. Bove, J. M. Durán-Vila, Núria |
| author_browse | Ben Mahamoud, O. Bove, J. M. Daros, José A. Durán-Vila, Núria Fadda, Z. Flores, R. Marques, Jorge Namsi, A. Takrouni, M. L. Zouba, Anis |
| author_facet | Namsi, A. Marques, Jorge Fadda, Z. Takrouni, M. L. Ben Mahamoud, O. Zouba, Anis Daros, José A. Flores, R. Bove, J. M. Durán-Vila, Núria |
| author_sort | Namsi, A. |
| collection | ReDivia |
| description | The “Maladie des feuilles cassantes” (MFC) or “Brittle leaf disease” of date palms is associated with the accumulation of two populations of small, chloroplast-encoded RNAs. A plasmid vector containing a cDNA with partial sequences of both of these RNA populations was used to synthesize a DIG-labeled bifunctional probe by PCR. The probe has been tested to detect, by molecular hybridization, MFC-associated RNAs from dsRNA-enriched palm leaflet preparations. Leaflet samples from MFC-affected date palm trees consistently gave a positive hybridization signal regardless of the date palm cultivar, severity of symptoms, or geographical location, whereas samples from date palm trees affected by other biotic and abiotic stresses tested negative. The assay is specific for MFC and can be used for early diagnostic purposes. |
| format | Artículo |
| id | ReDivia4199 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia41992025-04-25T14:42:32Z Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs Namsi, A. Marques, Jorge Fadda, Z. Takrouni, M. L. Ben Mahamoud, O. Zouba, Anis Daros, José A. Flores, R. Bove, J. M. Durán-Vila, Núria The “Maladie des feuilles cassantes” (MFC) or “Brittle leaf disease” of date palms is associated with the accumulation of two populations of small, chloroplast-encoded RNAs. A plasmid vector containing a cDNA with partial sequences of both of these RNA populations was used to synthesize a DIG-labeled bifunctional probe by PCR. The probe has been tested to detect, by molecular hybridization, MFC-associated RNAs from dsRNA-enriched palm leaflet preparations. Leaflet samples from MFC-affected date palm trees consistently gave a positive hybridization signal regardless of the date palm cultivar, severity of symptoms, or geographical location, whereas samples from date palm trees affected by other biotic and abiotic stresses tested negative. The assay is specific for MFC and can be used for early diagnostic purposes. 2017-06-01T10:09:34Z 2017-06-01T10:09:34Z 2006 DEC 2006 article Namsi, A., Marques, J., Fadda, Z., Takrouni, M.L., Ben Mahamoud, O., Zouba, A. et al. (2006). Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs. Molecular and cellular probes, 20(6), 366-370. 0890-8508 http://hdl.handle.net/20.500.11939/4199 10.1016/j.mcp.2006.05.001 en openAccess Impreso |
| spellingShingle | Namsi, A. Marques, Jorge Fadda, Z. Takrouni, M. L. Ben Mahamoud, O. Zouba, Anis Daros, José A. Flores, R. Bove, J. M. Durán-Vila, Núria Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title | Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title_full | Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title_fullStr | Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title_full_unstemmed | Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title_short | Diagnosis of "maladie des feuilles cassantes" or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs |
| title_sort | diagnosis of maladie des feuilles cassantes or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded rnas |
| url | http://hdl.handle.net/20.500.11939/4199 |
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