Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees
A proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. The aim of the study was to find out more about the molecular mechanism relating to alternate bearing in Citrus and its relationship wi...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4191 |
| _version_ | 1855032080216358912 |
|---|---|
| author | Munoz-Fambuena, Natalia Mesejo, Carlos Reig, Carmina Agustí, Manuel Tarraga, Susana Lison, Purificacion Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. |
| author_browse | Agustí, Manuel González-Mas, María C. Iglesias, Domingo J. Lison, Purificacion Mesejo, Carlos Munoz-Fambuena, Natalia Primo-Millo, Eduardo Reig, Carmina Tarraga, Susana |
| author_facet | Munoz-Fambuena, Natalia Mesejo, Carlos Reig, Carmina Agustí, Manuel Tarraga, Susana Lison, Purificacion Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. |
| author_sort | Munoz-Fambuena, Natalia |
| collection | ReDivia |
| description | A proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. The aim of the study was to find out more about the molecular mechanism relating to alternate bearing in Citrus and its relationship with flowering. The ‘Moncada’ variety (Clementine ‘Oroval’ x ‘Kara’ mandarin), displaying remarkable behaviour in alternate production, was used in this study. From 2D DIGE gel, 192 spots were isolated: 97 showed increased expression in the off-crop buds as compared to the on-crop buds, while 95 exhibited enhanced expression in the on-crop buds versus the off-crop buds. These spots were identified by MALDI-MS or LC–MS–MS. The largest groups of proteins up-expressed in the off-crop buds were the proteins involved in carbohydrate and amino acid metabolism, and the proteins expressed in response to stimuli such as reactive oxygen species. The largest groups of proteins up-expressed in the on-crop buds were related to primary metabolism, oxidative stress and defence responses. Depending on their function, some of these proteins can stimulate the flowering, such as fructose-bisphosphate aldolase or leucine-rich repeat transmembrane protein kinase, while others can inhibit it, such as cytochrome c oxidase subunit II. Twenty-two other proteins with unknown functions were up-expressed in the on- or off-crop buds. |
| format | article |
| id | ReDivia4191 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia41912025-04-25T14:42:30Z Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees Munoz-Fambuena, Natalia Mesejo, Carlos Reig, Carmina Agustí, Manuel Tarraga, Susana Lison, Purificacion Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. A proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. The aim of the study was to find out more about the molecular mechanism relating to alternate bearing in Citrus and its relationship with flowering. The ‘Moncada’ variety (Clementine ‘Oroval’ x ‘Kara’ mandarin), displaying remarkable behaviour in alternate production, was used in this study. From 2D DIGE gel, 192 spots were isolated: 97 showed increased expression in the off-crop buds as compared to the on-crop buds, while 95 exhibited enhanced expression in the on-crop buds versus the off-crop buds. These spots were identified by MALDI-MS or LC–MS–MS. The largest groups of proteins up-expressed in the off-crop buds were the proteins involved in carbohydrate and amino acid metabolism, and the proteins expressed in response to stimuli such as reactive oxygen species. The largest groups of proteins up-expressed in the on-crop buds were related to primary metabolism, oxidative stress and defence responses. Depending on their function, some of these proteins can stimulate the flowering, such as fructose-bisphosphate aldolase or leucine-rich repeat transmembrane protein kinase, while others can inhibit it, such as cytochrome c oxidase subunit II. Twenty-two other proteins with unknown functions were up-expressed in the on- or off-crop buds. 2017-06-01T10:09:33Z 2017-06-01T10:09:33Z 2013 DEC 2013 article Munoz-Fambuena, N., Mesejo, C., Reig, Carmina, Agusti, M., Tarraga, S., Lison, Purificacion, Iglesias, D.J., Primo-Millo, E., Gonzalez-Mas, M.C. (2013). Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees. Plant Physiology and Biochemistry, 73, 41-55. 0981-9428 http://hdl.handle.net/20.500.11939/4191 10.1016/j.plaphy.2013.08.008 en openAccess Impreso |
| spellingShingle | Munoz-Fambuena, Natalia Mesejo, Carlos Reig, Carmina Agustí, Manuel Tarraga, Susana Lison, Purificacion Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title | Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title_full | Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title_fullStr | Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title_full_unstemmed | Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title_short | Proteomic study of 'Moncada' mandarin buds from on- versus off-crop trees |
| title_sort | proteomic study of moncada mandarin buds from on versus off crop trees |
| url | http://hdl.handle.net/20.500.11939/4191 |
| work_keys_str_mv | AT munozfambuenanatalia proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT mesejocarlos proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT reigcarmina proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT agustimanuel proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT tarragasusana proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT lisonpurificacion proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT iglesiasdomingoj proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT primomilloeduardo proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees AT gonzalezmasmariac proteomicstudyofmoncadamandarinbudsfromonversusoffcroptrees |