First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface ste...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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American Phytopathological Society
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9790 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN https://doi.org/10.1094/PDIS-11-15-1298-PDN |
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| author | Conforto, Erica Cinthia Bernanrdi Lima, Nelson Garcete-Gómez, J. M. Câmara, M. P. S. Michereff, S. J. |
| author_browse | Bernanrdi Lima, Nelson Conforto, Erica Cinthia Câmara, M. P. S. Garcete-Gómez, J. M. Michereff, S. J. |
| author_facet | Conforto, Erica Cinthia Bernanrdi Lima, Nelson Garcete-Gómez, J. M. Câmara, M. P. S. Michereff, S. J. |
| author_sort | Conforto, Erica Cinthia |
| collection | INTA Digital |
| description | Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608). |
| format | info:ar-repo/semantics/artículo |
| id | INTA9790 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA97902021-07-13T10:42:26Z First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil Conforto, Erica Cinthia Bernanrdi Lima, Nelson Garcete-Gómez, J. M. Câmara, M. P. S. Michereff, S. J. Opuntia Brasil Opuntia Ficus Indica Cladodes Cladodios Prickly Pear Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608). Instituto de Patología Vegetal Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernardi Lima, Nelson. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil Fil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil Fil: Câmara, M. P. S. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil Fil: Michereff, S. J. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil 2021-07-13T10:29:36Z 2021-07-13T10:29:36Z 2016-03-18 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9790 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN 0191-2917 1943-7692 (online) https://doi.org/10.1094/PDIS-11-15-1298-PDN eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Plant Disease 100 (6) : 1238 (June 2016) |
| spellingShingle | Opuntia Brasil Opuntia Ficus Indica Cladodes Cladodios Prickly Pear Conforto, Erica Cinthia Bernanrdi Lima, Nelson Garcete-Gómez, J. M. Câmara, M. P. S. Michereff, S. J. First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title | First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title_full | First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title_fullStr | First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title_full_unstemmed | First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title_short | First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil |
| title_sort | first report of cladode brown spot in cactus prickly pear caused by neofusicoccum batangarum in brazil |
| topic | Opuntia Brasil Opuntia Ficus Indica Cladodes Cladodios Prickly Pear |
| url | http://hdl.handle.net/20.500.12123/9790 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN https://doi.org/10.1094/PDIS-11-15-1298-PDN |
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