Sample size for assessment of cladode brown spot in prickly pear cactus
Cladode brown spot (CBS) is an important disease of prickly pear cactus (Nopalea cochenillifera) in Brazil. No standard method exists for sampling in the field. A study was conducted in 30 fields of prickly pear (cv. Miúda) in Pernambuco state, Brazil, to determine the optimal sample size to assess...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/9787 https://link.springer.com/article/10.1007/s10658-017-1213-x https://doi.org/10.1007/s10658-017-1213-x |
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| author | Garcete-Gómez, José María Conforto, Erica Cinthia Domínguez-Monge, Santiago Flores-Sánchez, Jorge Luis Mora-Aguilera, Gustavo Michereff, Sami Jorge |
| author_browse | Conforto, Erica Cinthia Domínguez-Monge, Santiago Flores-Sánchez, Jorge Luis Garcete-Gómez, José María Michereff, Sami Jorge Mora-Aguilera, Gustavo |
| author_facet | Garcete-Gómez, José María Conforto, Erica Cinthia Domínguez-Monge, Santiago Flores-Sánchez, Jorge Luis Mora-Aguilera, Gustavo Michereff, Sami Jorge |
| author_sort | Garcete-Gómez, José María |
| collection | INTA Digital |
| description | Cladode brown spot (CBS) is an important disease of prickly pear cactus (Nopalea cochenillifera) in Brazil. No standard method exists for sampling in the field. A study was conducted in 30 fields of prickly pear (cv. Miúda) in Pernambuco state, Brazil, to determine the optimal sample size to assess severity of CBS. In each field, a 0.3 ha (45 × 68 m) test area consisting of 30 rows and 45 plants per row (1350 plants per test area) was arbitrarily chosen. In each test area, 50 plants were sampled using a systematic method and the disease severity was assessed with the aid of a standard area diagram set on 2, 4 and 6 cladodes/plant. The spatial pattern of the disease was examined by spatial autocorrelation analysis and the optimal sample sizes were determined based on three levels of error (5, 10 and 20%). CBS severity in the fields ranged from 0.4 to 8.7%. In 15 fields the disease severity of CBS was >4.0% and in 20 fields the spatial pattern of CBS was aggregated. There was a positive correlation (r = 0.59, P = 0.0001) between disease severity and strength of aggregation, and a negative correlation (r = −0.39; P = 0.0001) between disease severity and sample size (number of plants). There was no significant effect (P = 0.3) of the number of cladodes assessed per plant (2, 4 or 6 cladodes) on sample size. Considering a sample of two cladodes per plant and an acceptable error of 5, 10 or 20%, the optimal sample size was 253, 63 and 16 plants, respectively, for each 0.3 ha of cultivated field. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9787 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA97872021-07-12T17:41:39Z Sample size for assessment of cladode brown spot in prickly pear cactus Garcete-Gómez, José María Conforto, Erica Cinthia Domínguez-Monge, Santiago Flores-Sánchez, Jorge Luis Mora-Aguilera, Gustavo Michereff, Sami Jorge Epidemiology Sampling Cladodes Opuntia Epidemiología Muestreo Cladodios Nopalea Cochenillifera Prickly Pear Cladode brown spot (CBS) is an important disease of prickly pear cactus (Nopalea cochenillifera) in Brazil. No standard method exists for sampling in the field. A study was conducted in 30 fields of prickly pear (cv. Miúda) in Pernambuco state, Brazil, to determine the optimal sample size to assess severity of CBS. In each field, a 0.3 ha (45 × 68 m) test area consisting of 30 rows and 45 plants per row (1350 plants per test area) was arbitrarily chosen. In each test area, 50 plants were sampled using a systematic method and the disease severity was assessed with the aid of a standard area diagram set on 2, 4 and 6 cladodes/plant. The spatial pattern of the disease was examined by spatial autocorrelation analysis and the optimal sample sizes were determined based on three levels of error (5, 10 and 20%). CBS severity in the fields ranged from 0.4 to 8.7%. In 15 fields the disease severity of CBS was >4.0% and in 20 fields the spatial pattern of CBS was aggregated. There was a positive correlation (r = 0.59, P = 0.0001) between disease severity and strength of aggregation, and a negative correlation (r = −0.39; P = 0.0001) between disease severity and sample size (number of plants). There was no significant effect (P = 0.3) of the number of cladodes assessed per plant (2, 4 or 6 cladodes) on sample size. Considering a sample of two cladodes per plant and an acceptable error of 5, 10 or 20%, the optimal sample size was 253, 63 and 16 plants, respectively, for each 0.3 ha of cultivated field. Instituto de Patología Vegetal Fil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Domínguez-Monge, Santiago. Instituto de Fitosanidad. Colegio de Postgraduados. Fitopatología; México Fil: Flores-Sánchez, Jorge Luis. Instituto de Fitosanidad. Colegio de Postgraduados. Fitopatología; México Fil: Mora-Aguilera, Gustavo. Instituto de Fitosanidad. Colegio de Postgraduados. Fitopatología; México Fil: Michereff, Sami Jorge. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil 2021-07-12T17:24:12Z 2021-07-12T17:24:12Z 2017-03-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9787 https://link.springer.com/article/10.1007/s10658-017-1213-x 0929-1873 1573-8469 (online) https://doi.org/10.1007/s10658-017-1213-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology 149 : 759-763 (2017) |
| spellingShingle | Epidemiology Sampling Cladodes Opuntia Epidemiología Muestreo Cladodios Nopalea Cochenillifera Prickly Pear Garcete-Gómez, José María Conforto, Erica Cinthia Domínguez-Monge, Santiago Flores-Sánchez, Jorge Luis Mora-Aguilera, Gustavo Michereff, Sami Jorge Sample size for assessment of cladode brown spot in prickly pear cactus |
| title | Sample size for assessment of cladode brown spot in prickly pear cactus |
| title_full | Sample size for assessment of cladode brown spot in prickly pear cactus |
| title_fullStr | Sample size for assessment of cladode brown spot in prickly pear cactus |
| title_full_unstemmed | Sample size for assessment of cladode brown spot in prickly pear cactus |
| title_short | Sample size for assessment of cladode brown spot in prickly pear cactus |
| title_sort | sample size for assessment of cladode brown spot in prickly pear cactus |
| topic | Epidemiology Sampling Cladodes Opuntia Epidemiología Muestreo Cladodios Nopalea Cochenillifera Prickly Pear |
| url | http://hdl.handle.net/20.500.12123/9787 https://link.springer.com/article/10.1007/s10658-017-1213-x https://doi.org/10.1007/s10658-017-1213-x |
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