Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases aff...
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2021
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9785 https://www.sciencedirect.com/science/article/pii/S0261219421002027 https://doi.org/10.1016/j.cropro.2021.105732 |
| _version_ | 1855484460265373696 |
|---|---|
| author | Bernanrdi Lima, Nelson Conforto, Erica Cinthia Serri, Dannae Lilia Campilongo Mancilla, Ernesto Javier Stenglein, Sebastian Vargas Gil, Silvina |
| author_browse | Bernanrdi Lima, Nelson Campilongo Mancilla, Ernesto Javier Conforto, Erica Cinthia Serri, Dannae Lilia Stenglein, Sebastian Vargas Gil, Silvina |
| author_facet | Bernanrdi Lima, Nelson Conforto, Erica Cinthia Serri, Dannae Lilia Campilongo Mancilla, Ernesto Javier Stenglein, Sebastian Vargas Gil, Silvina |
| author_sort | Bernanrdi Lima, Nelson |
| collection | INTA Digital |
| description | Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen. |
| format | Artículo |
| id | INTA9785 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA97852021-07-12T17:14:19Z Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina Bernanrdi Lima, Nelson Conforto, Erica Cinthia Serri, Dannae Lilia Campilongo Mancilla, Ernesto Javier Stenglein, Sebastian Vargas Gil, Silvina Medicago Sativa Colletotrichum Argentina Anthracnosis Phylogeny Filogenia Antracnosis Molecular Phylogeny Plant Pathogen Alfalfa Lucerne Córdoba, Argentina Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen. Instituto de Patología Vegetal Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Campilongo Mancilla, Ernesto Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Campilongo Mancilla, Ernesto Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Stenglein, Sebastian. Laboratorio de Biología Functional y Biotecnología (BIOLAB). Unidad Ejecutora Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2021-07-12T16:43:02Z 2021-07-12T16:43:02Z 2021-06-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9785 https://www.sciencedirect.com/science/article/pii/S0261219421002027 0261-2194 https://doi.org/10.1016/j.cropro.2021.105732 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes info:eu-repo/semantics/restrictedAccess application/pdf Córdoba .......... (province) (World, South America, Argentina) 1001169 Elsevier Crop Protection 148 : 105732 ( 2021) |
| spellingShingle | Medicago Sativa Colletotrichum Argentina Anthracnosis Phylogeny Filogenia Antracnosis Molecular Phylogeny Plant Pathogen Alfalfa Lucerne Córdoba, Argentina Bernanrdi Lima, Nelson Conforto, Erica Cinthia Serri, Dannae Lilia Campilongo Mancilla, Ernesto Javier Stenglein, Sebastian Vargas Gil, Silvina Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title | Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title_full | Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title_fullStr | Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title_full_unstemmed | Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title_short | Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina |
| title_sort | anthracnose on alfalfa caused by colletotrichum americae borealis in cordoba province argentina |
| topic | Medicago Sativa Colletotrichum Argentina Anthracnosis Phylogeny Filogenia Antracnosis Molecular Phylogeny Plant Pathogen Alfalfa Lucerne Córdoba, Argentina |
| url | http://hdl.handle.net/20.500.12123/9785 https://www.sciencedirect.com/science/article/pii/S0261219421002027 https://doi.org/10.1016/j.cropro.2021.105732 |
| work_keys_str_mv | AT bernanrdilimanelson anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina AT confortoericacinthia anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina AT serridannaelilia anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina AT campilongomancillaernestojavier anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina AT stengleinsebastian anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina AT vargasgilsilvina anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina |