Characterization of grapevine wood microbiome through a metatranscriptomic approach
Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture....
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9743 https://link.springer.com/article/10.1007/s00248-021-01801-z https://doi.org/10.1007/s00248-021-01801-z |
| _version_ | 1855484452825726976 |
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| author | Paolinelli, Marcos Escoriaza, Maria Georgina Cesari, Cecilia Alicia Garcia Lampasona, Sandra Claudia Hernandez Martinez, Rufina |
| author_browse | Cesari, Cecilia Alicia Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Hernandez Martinez, Rufina Paolinelli, Marcos |
| author_facet | Paolinelli, Marcos Escoriaza, Maria Georgina Cesari, Cecilia Alicia Garcia Lampasona, Sandra Claudia Hernandez Martinez, Rufina |
| author_sort | Paolinelli, Marcos |
| collection | INTA Digital |
| description | Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its
advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM)
significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease,
but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood
microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic
study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic
or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with
more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected.
Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance
of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with
foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial
interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification
for specific taxa. |
| format | Artículo |
| id | INTA9743 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA97432021-07-06T18:00:14Z Characterization of grapevine wood microbiome through a metatranscriptomic approach Paolinelli, Marcos Escoriaza, Maria Georgina Cesari, Cecilia Alicia Garcia Lampasona, Sandra Claudia Hernandez Martinez, Rufina Vid Enfermedades Fungosas Microorganismos ADN Phellinus Basidiomycota Grapevines Fungal Diseases Microorganisms DNA Sequence Hoja de Malvón Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease, but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected. Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification for specific taxa. EEA Mendoza Fil: Paolinelli, Marcos. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Escoriaza, Maria Georgina. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Cesari, Cecilia Alicia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias, Instituto de Biología Agrícola de Mendoza (IBAM), Mendoza, Argentina Fil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza; Argentina Fil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE); México 2021-07-06T17:38:32Z 2021-07-06T17:38:32Z 2021-06-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9743 https://link.springer.com/article/10.1007/s00248-021-01801-z 0095-3628 1432-184X https://doi.org/10.1007/s00248-021-01801-z eng info:eu-repograntAgreement/INTA/MZASJ-1251101/AR./Desarrollo territorial del oasis norte de Mendoza. info:eu-repo/semantics/restrictedAccess application/pdf Springer Microbial Ecology (30 June 2021) |
| spellingShingle | Vid Enfermedades Fungosas Microorganismos ADN Phellinus Basidiomycota Grapevines Fungal Diseases Microorganisms DNA Sequence Hoja de Malvón Paolinelli, Marcos Escoriaza, Maria Georgina Cesari, Cecilia Alicia Garcia Lampasona, Sandra Claudia Hernandez Martinez, Rufina Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title | Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title_full | Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title_fullStr | Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title_full_unstemmed | Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title_short | Characterization of grapevine wood microbiome through a metatranscriptomic approach |
| title_sort | characterization of grapevine wood microbiome through a metatranscriptomic approach |
| topic | Vid Enfermedades Fungosas Microorganismos ADN Phellinus Basidiomycota Grapevines Fungal Diseases Microorganisms DNA Sequence Hoja de Malvón |
| url | http://hdl.handle.net/20.500.12123/9743 https://link.springer.com/article/10.1007/s00248-021-01801-z https://doi.org/10.1007/s00248-021-01801-z |
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