Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells
Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform wi...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
ACS Publications
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9742 https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.1c00048 https://doi.org/10.1021/acs.molpharmaceut.1c00048 |
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| author | Pappalardo, Juan Sebastián Salmaso, Stefano Levchenko, Tatyana S. Mastrotto, Francesca Bersani, Sara Vermeulen, Monica Ghersa, Federica Quattrocchi, Valeria Zamorano, Patricia Ines Hartner, William C. Toniutti, Micaela Musacchio, Tiziana Torchilin, Vladimir P. |
| author_browse | Bersani, Sara Ghersa, Federica Hartner, William C. Levchenko, Tatyana S. Mastrotto, Francesca Musacchio, Tiziana Pappalardo, Juan Sebastián Quattrocchi, Valeria Salmaso, Stefano Toniutti, Micaela Torchilin, Vladimir P. Vermeulen, Monica Zamorano, Patricia Ines |
| author_facet | Pappalardo, Juan Sebastián Salmaso, Stefano Levchenko, Tatyana S. Mastrotto, Francesca Bersani, Sara Vermeulen, Monica Ghersa, Federica Quattrocchi, Valeria Zamorano, Patricia Ines Hartner, William C. Toniutti, Micaela Musacchio, Tiziana Torchilin, Vladimir P. |
| author_sort | Pappalardo, Juan Sebastián |
| collection | INTA Digital |
| description | Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo can boost the immunogenicity, resulting in a longlasting
immunity. In this work, we aim to characterize the α1′,2-mannobiose derivative, which is key in the nanovaccine platform. This DC-targeting strategy takes advantage of the specific receptor known as DC-SIGN and exploits its capacity to bind α1,2-mannobiose that
is present at terminal ends of oligosaccharides in certain viruses, bacteria, and other pathogens. The oxidative conjugation of α1′,2-mannobiose to NH2-PEG2kDa-DSPE allowed us to preserve the chemical structure of the non-reducing mannose of the disaccharide and the OH groups and the stereochemistry of all carbons of the reducing mannose involved in the binding to DC-SIGN. Here, we show specific targeting to DC-SIGN of decorated micelles incubated with the Raji/DC-SIGN cell line and uptake of targeted liposomes that took place in human, bovine, mouse, and teleost fish DCs in vitro, by flow cytometry.
Specific targeting was found in all cultures, demonstrating a species-non-specific avidity for this ligand, which opens up the possibility
of using this nanoplatform to develop new vaccines for various species, including humans. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9742 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | ACS Publications |
| publisherStr | ACS Publications |
| record_format | dspace |
| spelling | INTA97422021-07-06T17:33:11Z Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells Pappalardo, Juan Sebastián Salmaso, Stefano Levchenko, Tatyana S. Mastrotto, Francesca Bersani, Sara Vermeulen, Monica Ghersa, Federica Quattrocchi, Valeria Zamorano, Patricia Ines Hartner, William C. Toniutti, Micaela Musacchio, Tiziana Torchilin, Vladimir P. Nanomedicina Vacuna Antígenos Respuesta Inmunológica Nanomedicine Vaccines Antigens Immune Response Nanovacuna Células Dendríticas Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo can boost the immunogenicity, resulting in a longlasting immunity. In this work, we aim to characterize the α1′,2-mannobiose derivative, which is key in the nanovaccine platform. This DC-targeting strategy takes advantage of the specific receptor known as DC-SIGN and exploits its capacity to bind α1,2-mannobiose that is present at terminal ends of oligosaccharides in certain viruses, bacteria, and other pathogens. The oxidative conjugation of α1′,2-mannobiose to NH2-PEG2kDa-DSPE allowed us to preserve the chemical structure of the non-reducing mannose of the disaccharide and the OH groups and the stereochemistry of all carbons of the reducing mannose involved in the binding to DC-SIGN. Here, we show specific targeting to DC-SIGN of decorated micelles incubated with the Raji/DC-SIGN cell line and uptake of targeted liposomes that took place in human, bovine, mouse, and teleost fish DCs in vitro, by flow cytometry. Specific targeting was found in all cultures, demonstrating a species-non-specific avidity for this ligand, which opens up the possibility of using this nanoplatform to develop new vaccines for various species, including humans. Estación Experimental Agropecuaria Bariloche Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Pappalardo, Juan Sebastián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Salmaso, Stefano. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia Fil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Mastrotto, Francesca. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia Fil: Bersani, Sara. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia Fil: Vermeulen, Monica. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Vermeulen, Monica. Academia Nacional de Medicina. Instituto de Medicina Experimental; Argentina Fil: Ghersa, Federica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina Fil: Ghersa, Federica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Ghersa, Federica. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Toniutti, Micaela. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Musacchio, Tiziana. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos 2021-07-06T17:11:03Z 2021-07-06T17:11:03Z 2021-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9742 https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.1c00048 1543-8384 https://doi.org/10.1021/acs.molpharmaceut.1c00048 eng info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/restrictedAccess application/pdf ACS Publications Molecular pharmaceutics 18 (7) : 2540-2555 (Julio 2021) |
| spellingShingle | Nanomedicina Vacuna Antígenos Respuesta Inmunológica Nanomedicine Vaccines Antigens Immune Response Nanovacuna Células Dendríticas Pappalardo, Juan Sebastián Salmaso, Stefano Levchenko, Tatyana S. Mastrotto, Francesca Bersani, Sara Vermeulen, Monica Ghersa, Federica Quattrocchi, Valeria Zamorano, Patricia Ines Hartner, William C. Toniutti, Micaela Musacchio, Tiziana Torchilin, Vladimir P. Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title_full | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title_fullStr | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title_full_unstemmed | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title_short | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells |
| title_sort | characterization of a nanovaccine platform based on an α1 2 mannobiose derivative shows species non specific targeting to human bovine mouse and teleost fish dendritic cells |
| topic | Nanomedicina Vacuna Antígenos Respuesta Inmunológica Nanomedicine Vaccines Antigens Immune Response Nanovacuna Células Dendríticas |
| url | http://hdl.handle.net/20.500.12123/9742 https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.1c00048 https://doi.org/10.1021/acs.molpharmaceut.1c00048 |
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