First report of Papaya ringspot virus infecting Carica papaya in Argentina
Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron micr...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9708 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3691 http://dx.doi.org/10.4454/JPP.V98I3.017 |
| _version_ | 1855036260816519168 |
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| author | Cabrera Mederos, Dariel Dal Zotto, Angelica Galdeano, E. Portal, O. Giolitti, Fabian |
| author_browse | Cabrera Mederos, Dariel Dal Zotto, Angelica Galdeano, E. Giolitti, Fabian Portal, O. |
| author_facet | Cabrera Mederos, Dariel Dal Zotto, Angelica Galdeano, E. Portal, O. Giolitti, Fabian |
| author_sort | Cabrera Mederos, Dariel |
| collection | INTA Digital |
| description | Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9708 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| publisherStr | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| record_format | dspace |
| spelling | INTA97082021-07-01T12:28:44Z First report of Papaya ringspot virus infecting Carica papaya in Argentina Cabrera Mederos, Dariel Dal Zotto, Angelica Galdeano, E. Portal, O. Giolitti, Fabian Carica Papaya Argentina Carica Papaya Potyvirus Virus Viruses Papaya Ringspot Virus Papayas Papaya Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas. Instituto de Patología Vegetal Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cuba Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2021-07-01T12:16:17Z 2021-07-01T12:16:17Z 2016-06-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9708 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3691 1125-4653 2239-7264 (online) http://dx.doi.org/10.4454/JPP.V98I3.017 eng info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari Journal of Plant Pathology 98 (3) : 687 (2016) |
| spellingShingle | Carica Papaya Argentina Carica Papaya Potyvirus Virus Viruses Papaya Ringspot Virus Papayas Papaya Cabrera Mederos, Dariel Dal Zotto, Angelica Galdeano, E. Portal, O. Giolitti, Fabian First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title | First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title_full | First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title_fullStr | First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title_full_unstemmed | First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title_short | First report of Papaya ringspot virus infecting Carica papaya in Argentina |
| title_sort | first report of papaya ringspot virus infecting carica papaya in argentina |
| topic | Carica Papaya Argentina Carica Papaya Potyvirus Virus Viruses Papaya Ringspot Virus Papayas Papaya |
| url | http://hdl.handle.net/20.500.12123/9708 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3691 http://dx.doi.org/10.4454/JPP.V98I3.017 |
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