Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought
Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expres...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9683 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w https://doi.org/10.1186/s43141-021-00171-w |
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| author | Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel |
| author_browse | Abeledo, Leonor Gabriela Beznec, Ailin Blumwald, Eduardo Bossio, Adrian Ezequiel Decima Oneto, Cecilia Andrea Diaz Paleo, Antonio Horacio Elizondo, Matias Faccio, Paula Daniela Garibotto, María De Belén Gonzalez, Germán Andrés Lewi, Dalia Marcela Llorente, Berta Miralles, Daniel J. Moreyra, Federico Ruiz, Monica Beatriz |
| author_facet | Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel |
| author_sort | Beznec, Ailin |
| collection | INTA Digital |
| description | Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions.
Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions.
Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9683 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA96832022-08-09T14:03:56Z Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress Background: The heterologous expression of isopentenyl transferase (IPT) under the transcriptional control of the senescence-associated receptor-like kinase (SARK) promoter delayed cellular senescence and, through it, increased drought tolerance in plants. To evaluate the effect of pSARK::IPT expression in bread wheat, six independent transgenic events were obtained through the biolistic method and evaluated transgene expression, phenology, grain yield and physiological biomass components in plants grown under both drought and well-irrigating conditions. Experiments were performed at different levels: (i) pots and (ii) microplots inside a biosafety greenhouse, as well as under (iii) field conditions. Results: Two transgenic events, called TR1 and TR4, outperformed the wild-type control under drought conditions. Transgenic plants showed higher yield under both greenhouse and field conditions, which was positively correlated to grain number (given by more spikes and grains per spike) than wild type. Interestingly, this yield advantage of the transgenic events was observed under both drought and well-watered conditions. Conclusions: The results obtained allow us to conclude that the SARK promoter-regulated expression of the IPT gene in bread wheat not only reduced the yield penalty produced by water stress but also led to improved productivity under well-watered conditions. EEA Pergamino Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina Fil: Miralles, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina Fil: Abeledo, Leonor G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Cerealicultura; Argentina Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Agrobiotecnología; Argentina Fil: Décima Oneto, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garibotto, María De Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Garibotto, María De Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Germán Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina Fil: Moreyra, Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; Argentina Fil: Elizondo, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Elizondo, Matias. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina Fil: Ruiz, Mónica Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina Fil: Ruiz, Mónica Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Departamento de Agronomía. Unidad Integrada INTA-UNSJ; Argentina Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos Fil: Llorente, Berta. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina Fil: Diaz Paleo, Antonio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Laboratorio Biotecnología; Argentina Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2021-06-29T12:14:44Z 2021-06-29T12:14:44Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9683 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w https://doi.org/10.1186/s43141-021-00171-w eng info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Journal of Genetic Engineering and Biotechnology 19 : 1-17. (May 2021) |
| spellingShingle | Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress Beznec, Ailin Faccio, Paula Daniela Miralles, Daniel J. Abeledo, Leonor Gabriela Decima Oneto, Cecilia Andrea Garibotto, María De Belén Gonzalez, Germán Andrés Moreyra, Federico Elizondo, Matias Ruiz, Monica Beatriz Lewi, Dalia Marcela Blumwald, Eduardo Llorente, Berta Diaz Paleo, Antonio Horacio Bossio, Adrian Ezequiel Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title | Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title_full | Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title_fullStr | Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title_full_unstemmed | Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title_short | Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought |
| title_sort | stress induced expression of ipt gene in transgenic wheat reduces grain yield penalty under drought |
| topic | Trigo Genética Citoquininas Estrés de Sequia Wheat Genetics Cytokinins Drought Stress |
| url | http://hdl.handle.net/20.500.12123/9683 https://jgeb.springeropen.com/articles/10.1186/s43141-021-00171-w https://doi.org/10.1186/s43141-021-00171-w |
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