Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina
Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, P...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Microbiology Society
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9668 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003704 https://doi.org/10.1099/ijsem.0.003704 |
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| author | Fernandez, Franco Daniel Galdeano, Ernestina Conci, Luis Rogelio |
| author_browse | Conci, Luis Rogelio Fernandez, Franco Daniel Galdeano, Ernestina |
| author_facet | Fernandez, Franco Daniel Galdeano, Ernestina Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma
ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X)
B. subalternans, H. ovatifolium, and C.
maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B)
and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-(
B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas
infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9668 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Microbiology Society |
| publisherStr | Microbiology Society |
| record_format | dspace |
| spelling | INTA96682021-06-25T18:50:31Z Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina Fernandez, Franco Daniel Galdeano, Ernestina Conci, Luis Rogelio Phytoplasmas Weeds Phyllody Witches' Broom Classification Filodia Fitoplasmas Malezas Clasificación Argentina Phytoplasma Diversity Symptoms of phytoplasma infection were observed in different weed species, Bidens subalternans, Conyza bonariensis, Heterosperma ovatifolium and Conium maculatum, collected from diverse geographical regions in Argentina. To confirm the association of phytoplasma infection with symptomatic plants, PCR, RFLP and phylogenetic analyses based on 16S rRNA-encoding sequences were performed. In this work, we report the presence of phytoplasmas from group 16SrVII (subgroup 16VII-B) infecting C. bonariensis and B. subalternans and from group 16SrIII (subgroup 16SrIII-X) B. subalternans, H. ovatifolium, and C. maculatum. Phytoplasmas from the aster yellows group were detected infecting C. bonariensis and B. subalternans. Analysis of 16S rRNA-encoding genes revealed the presence of two distinct operons, rrnB (16SrI-B) and newly described rrnA, which is different from the reference RFLP patterns of all previously established 16SrI-subgroups. A single rp operon sequence analysis reveals the presence of simple infection and confirms a description of a novel subgroup. On the basis of these results we propose a designation of new subgroup 16SrI-( B/AJ) AJ (rp-AJ). To our knowledge, this is the first report of phytoplasmas infecting Bidens subalternans¸ Heterosperma ovatifolium and Conium maculatum. Instituto de Patología Vegetal Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2021-06-25T18:36:15Z 2021-06-25T18:36:15Z 2020-02-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9668 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003704 1466-5026 1466-5034 (online) https://doi.org/10.1099/ijsem.0.003704 eng info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina. info:eu-repograntAgreement/INTA/PNIND-1108071/AR./Estrategias de manejo de sistemas productivos resilientes. info:eu-repograntAgreement/INTA/PNIND-1108072/AR./Epidemiología de plagas y enfermedades en cultivos industriales con enfoque al desarrollo de estrategias de manejo integrado. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Microbiology Society International Journal of Systematic and Evolutionary Microbiology 70 (1) : 35-43 (2020) |
| spellingShingle | Phytoplasmas Weeds Phyllody Witches' Broom Classification Filodia Fitoplasmas Malezas Clasificación Argentina Phytoplasma Diversity Fernandez, Franco Daniel Galdeano, Ernestina Conci, Luis Rogelio Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title | Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title_full | Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title_fullStr | Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title_full_unstemmed | Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title_short | Phytoplasmas diversity and identification of new aster yellows subgroup (16SrI) associated with weed species in Argentina |
| title_sort | phytoplasmas diversity and identification of new aster yellows subgroup 16sri associated with weed species in argentina |
| topic | Phytoplasmas Weeds Phyllody Witches' Broom Classification Filodia Fitoplasmas Malezas Clasificación Argentina Phytoplasma Diversity |
| url | http://hdl.handle.net/20.500.12123/9668 https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003704 https://doi.org/10.1099/ijsem.0.003704 |
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