Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction...
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/9664 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272 https://doi.org/10.1111/ppa.13272 |
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| author | Zanini, Andrea Alejandra Di Feo, Liliana Del Valle Luna, Dario Fernando Paccioretti, Pablo Collavino, Agostina Rodriguez, Marianela |
| author_browse | Collavino, Agostina Di Feo, Liliana Del Valle Luna, Dario Fernando Paccioretti, Pablo Rodriguez, Marianela Zanini, Andrea Alejandra |
| author_facet | Zanini, Andrea Alejandra Di Feo, Liliana Del Valle Luna, Dario Fernando Paccioretti, Pablo Collavino, Agostina Rodriguez, Marianela |
| author_sort | Zanini, Andrea Alejandra |
| collection | INTA Digital |
| description | Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this
study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting,
respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter
phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive
chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield. |
| format | Artículo |
| id | INTA9664 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA96642021-06-25T16:36:04Z Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants Zanini, Andrea Alejandra Di Feo, Liliana Del Valle Luna, Dario Fernando Paccioretti, Pablo Collavino, Agostina Rodriguez, Marianela Biotic Stress Cassava Potexviruses Estrés Biótico Mandioca Cloroplasto Potexvirus Chloroplast Alteration CsCMV OJIP Test Potexvirus Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Di Feo, Liliana Del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Luna, Dario Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Paccioretti, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina Fil: Collavino, Agostina. Instituto Universitario de Formosa. Facultad de la Producción y del Medio Ambiente. Centro de Investigación y Transferencia de Formosa; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina 2021-06-25T16:16:52Z 2021-06-25T16:16:52Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9664 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272 0032-0862 1365-3059 (online) https://doi.org/10.1111/ppa.13272 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Pathology 70 (1) : 195-205 (January 2021) |
| spellingShingle | Biotic Stress Cassava Potexviruses Estrés Biótico Mandioca Cloroplasto Potexvirus Chloroplast Alteration CsCMV OJIP Test Potexvirus Zanini, Andrea Alejandra Di Feo, Liliana Del Valle Luna, Dario Fernando Paccioretti, Pablo Collavino, Agostina Rodriguez, Marianela Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title | Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title_full | Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title_fullStr | Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title_full_unstemmed | Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title_short | Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants |
| title_sort | cassava common mosaic virus infection causes alterations in chloroplast ultrastructure function and carbohydrate metabolism of cassava plants |
| topic | Biotic Stress Cassava Potexviruses Estrés Biótico Mandioca Cloroplasto Potexvirus Chloroplast Alteration CsCMV OJIP Test Potexvirus |
| url | http://hdl.handle.net/20.500.12123/9664 https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272 https://doi.org/10.1111/ppa.13272 |
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