First report of papaya ringspot virus w infecting momordica charantia in Cuba
During a survey conducted in July 2010, severe mosaic and deformations were observed on the leaves of cerasee (Momordica charantia) in Villa Clara, Cuba. The symptoms resembled those induced by Papaya ringspot virus (PRSV) in papaya (Carica papaya L.) plants (Olarte et al., 2011). Leaf samples were...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9627 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3282 http://dx.doi.org/10.4454/JPP.V97I1.041 |
| _version_ | 1855036246107095040 |
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| author | Cabrera Mederos, Dariel Giolitti, Fabian Portal, O. |
| author_browse | Cabrera Mederos, Dariel Giolitti, Fabian Portal, O. |
| author_facet | Cabrera Mederos, Dariel Giolitti, Fabian Portal, O. |
| author_sort | Cabrera Mederos, Dariel |
| collection | INTA Digital |
| description | During a survey conducted in July 2010, severe mosaic and deformations were observed on the leaves of cerasee (Momordica charantia) in Villa Clara, Cuba. The symptoms resembled those induced by Papaya ringspot virus (PRSV) in papaya (Carica papaya L.) plants (Olarte et al., 2011). Leaf samples were
ELISA positive when tested with a specific PRSV antiserum (Agdia, USA). The presence of PRSV was confirmed by the amplification of a 850 bp fragment by RT-PCR using primers specific to the PRSV coat protein gene (unpublished information). BLASTn analysis of the sequences from the RT-PCR amplified
product (GenBank accession No. KP019380) showed 94% identity with PRSV-W isolates from Citrullus lanatus in the USA (GenBank accession Nos. JN132439 to JN132457) and PRSV-P isolates (GenBank accession Nos. AF196839 and EF189736). Crude sap extracts from symptomatic M. charantia
leaf samples were mechanically inoculated onto Cucurbita moschata and papaya plants. Symptoms developed in the former but not in the latter plants, suggesting the presence of PRSV-W, since only PRSV-P is able to infect papaya plants (Romay et al., 2014). In inoculated plants, the presence of this
virus was determined by ELISA. In Cuba, the first molecular detection of PRSV-W was from Cucurbita pepo (Rodríguez-
Martínez, personal communication) and PRSV-P has only been reported in papaya plants (Portal et al., 2006). To our knowledge, this is the first report of PRSV-W infecting M. charantia in Cuba. This finding is relevant for the epidemiology, and management of this disease in cucurbits. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9627 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| publisherStr | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| record_format | dspace |
| spelling | INTA96272021-06-22T12:42:15Z First report of papaya ringspot virus w infecting momordica charantia in Cuba Cabrera Mederos, Dariel Giolitti, Fabian Portal, O. Papayas Momordica Virus de las Plantas Cucurbitaceae Momordica Charantia Plant Viruses Cuba Papaya During a survey conducted in July 2010, severe mosaic and deformations were observed on the leaves of cerasee (Momordica charantia) in Villa Clara, Cuba. The symptoms resembled those induced by Papaya ringspot virus (PRSV) in papaya (Carica papaya L.) plants (Olarte et al., 2011). Leaf samples were ELISA positive when tested with a specific PRSV antiserum (Agdia, USA). The presence of PRSV was confirmed by the amplification of a 850 bp fragment by RT-PCR using primers specific to the PRSV coat protein gene (unpublished information). BLASTn analysis of the sequences from the RT-PCR amplified product (GenBank accession No. KP019380) showed 94% identity with PRSV-W isolates from Citrullus lanatus in the USA (GenBank accession Nos. JN132439 to JN132457) and PRSV-P isolates (GenBank accession Nos. AF196839 and EF189736). Crude sap extracts from symptomatic M. charantia leaf samples were mechanically inoculated onto Cucurbita moschata and papaya plants. Symptoms developed in the former but not in the latter plants, suggesting the presence of PRSV-W, since only PRSV-P is able to infect papaya plants (Romay et al., 2014). In inoculated plants, the presence of this virus was determined by ELISA. In Cuba, the first molecular detection of PRSV-W was from Cucurbita pepo (Rodríguez- Martínez, personal communication) and PRSV-P has only been reported in papaya plants (Portal et al., 2006). To our knowledge, this is the first report of PRSV-W infecting M. charantia in Cuba. This finding is relevant for the epidemiology, and management of this disease in cucurbits. Instituto de Patología Vegetal Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cabrera Mederos, Dariel. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cuba Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cuba 2021-06-22T12:31:54Z 2021-06-22T12:31:54Z 2015-01-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9627 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3282 1125-4653 2239-7264 (online) http://dx.doi.org/10.4454/JPP.V97I1.041 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari Journal of Plant Pathology 97 (1) : 211 (2015) |
| spellingShingle | Papayas Momordica Virus de las Plantas Cucurbitaceae Momordica Charantia Plant Viruses Cuba Papaya Cabrera Mederos, Dariel Giolitti, Fabian Portal, O. First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title | First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title_full | First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title_fullStr | First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title_full_unstemmed | First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title_short | First report of papaya ringspot virus w infecting momordica charantia in Cuba |
| title_sort | first report of papaya ringspot virus w infecting momordica charantia in cuba |
| topic | Papayas Momordica Virus de las Plantas Cucurbitaceae Momordica Charantia Plant Viruses Cuba Papaya |
| url | http://hdl.handle.net/20.500.12123/9627 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3282 http://dx.doi.org/10.4454/JPP.V97I1.041 |
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