First report of raspberry bushy dwarf virus infecting raspberry in Argentina
In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9626 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896 http://dx.doi.org/10.4454/jpp.v99i2.3896 |
| _version_ | 1855036245917302784 |
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| author | Dal Zotto, Angelica Cardozo, Andrea Cabrera Mederos, Dariel Nome Docampo, Claudia Giolitti, Fabian Cobelo, Claudia Monica |
| author_browse | Cabrera Mederos, Dariel Cardozo, Andrea Cobelo, Claudia Monica Dal Zotto, Angelica Giolitti, Fabian Nome Docampo, Claudia |
| author_facet | Dal Zotto, Angelica Cardozo, Andrea Cabrera Mederos, Dariel Nome Docampo, Claudia Giolitti, Fabian Cobelo, Claudia Monica |
| author_sort | Dal Zotto, Angelica |
| collection | INTA Digital |
| description | In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in
commercial fields in parallel 42° in the Patagonia region of Argentina.
Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus.
RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with
Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is
the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9626 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| publisherStr | Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari |
| record_format | dspace |
| spelling | INTA96262021-06-18T18:40:28Z First report of raspberry bushy dwarf virus infecting raspberry in Argentina Dal Zotto, Angelica Cardozo, Andrea Cabrera Mederos, Dariel Nome Docampo, Claudia Giolitti, Fabian Cobelo, Claudia Monica Raspberries Dwarfism Rubus Idaeus Frambuesa Virus de las Plantas Raspberry In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production. Instituto de Patología Vegetal Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cobelo, Claudia Mónica. nstituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina 2021-06-18T18:22:43Z 2021-06-18T18:22:43Z 2017-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9626 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896 1125-4653 2239-7264 (online) http://dx.doi.org/10.4454/jpp.v99i2.3896 eng info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina. info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari Journal of Plant Pathology 99 (2) : 539 (2017) |
| spellingShingle | Raspberries Dwarfism Rubus Idaeus Frambuesa Virus de las Plantas Raspberry Dal Zotto, Angelica Cardozo, Andrea Cabrera Mederos, Dariel Nome Docampo, Claudia Giolitti, Fabian Cobelo, Claudia Monica First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title | First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title_full | First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title_fullStr | First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title_full_unstemmed | First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title_short | First report of raspberry bushy dwarf virus infecting raspberry in Argentina |
| title_sort | first report of raspberry bushy dwarf virus infecting raspberry in argentina |
| topic | Raspberries Dwarfism Rubus Idaeus Frambuesa Virus de las Plantas Raspberry |
| url | http://hdl.handle.net/20.500.12123/9626 http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896 http://dx.doi.org/10.4454/jpp.v99i2.3896 |
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