Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D
In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrat...
| Main Authors: | , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/960 http://online.liebertpub.com/doi/10.1089/vim.2016.0090 https://doi.org/10.1089/vim.2016.0090 |
| _version_ | 1855034736298164224 |
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| author | Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela |
| author_browse | Calamante, Gabriela Del Medico Zajac, Maria Paula Federico, Carlos Rodolfo Valera, Alejandro Rafael Zabal, Osvaldo Alfredo Zanetti, Flavia Adriana |
| author_facet | Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela |
| author_sort | Del Medico Zajac, Maria Paula |
| collection | INTA Digital |
| description | In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability
of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level. |
| format | info:ar-repo/semantics/artículo |
| id | INTA960 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9602023-08-29T12:15:14Z Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level. Inst. de Biotecnología Fil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esusy, María Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Federico, Carlos Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Valera, Alejandro Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Virología; Argentina Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2017-08-14T12:49:13Z 2017-08-14T12:49:13Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/960 http://online.liebertpub.com/doi/10.1089/vim.2016.0090 1557-8976 (Online) 0882-8245 (Print) https://doi.org/10.1089/vim.2016.0090 eng info:eu-repo/semantics/restrictedAccess application/pdf Viral immunology 30 (1) : 70-76. (2017) |
| spellingShingle | Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins Del Medico Zajac, Maria Paula Zanetti, Flavia Adriana Federico, Carlos Rodolfo Zabal, Osvaldo Alfredo Valera, Alejandro Rafael Calamante, Gabriela Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title | Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title_full | Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title_fullStr | Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title_full_unstemmed | Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title_short | Induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein D |
| title_sort | induction of both local immune response in mice and protection in a rabbit model by intranasal immunization with modified vaccinia ankara virus expressing a secreted form of bovine herpesvirus 1 glycoprotein d |
| topic | Enfermedades de los Animales Respuesta inmunológica Ratón Herpes Virus Bovino Glicoproteínas Immune response Animal Diseases Mice Bovine herpesvirus Glycoproteins |
| url | http://hdl.handle.net/20.500.12123/960 http://online.liebertpub.com/doi/10.1089/vim.2016.0090 https://doi.org/10.1089/vim.2016.0090 |
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