Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes

Chlamydia abortus infects livestock species worldwide and is the cause of enzootic abortion of ewes (EAE). In Europe, control of the disease is achieved using a live vaccine based on C. abortus 1B strain. Although the vaccine has been useful for controlling disease outbreaks, abortion events due to...

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Main Authors: Caspe, Sergio Gaston, Palarea-Albaladejo, Javier, Underwood, Clare, Livingstone, Morag, Wattegedera, Sean Ranjan, Milne, Elspeth, Sargison, Neil Donald, Chianini, Francesca, Longbottom, David
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: MDPI 2021
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/9561
https://www.mdpi.com/2076-0817/10/5/543
https://doi.org/10.3390/pathogens10050543
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author Caspe, Sergio Gaston
Palarea-Albaladejo, Javier
Underwood, Clare
Livingstone, Morag
Wattegedera, Sean Ranjan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
author_browse Caspe, Sergio Gaston
Chianini, Francesca
Livingstone, Morag
Longbottom, David
Milne, Elspeth
Palarea-Albaladejo, Javier
Sargison, Neil Donald
Underwood, Clare
Wattegedera, Sean Ranjan
author_facet Caspe, Sergio Gaston
Palarea-Albaladejo, Javier
Underwood, Clare
Livingstone, Morag
Wattegedera, Sean Ranjan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
author_sort Caspe, Sergio Gaston
collection INTA Digital
description Chlamydia abortus infects livestock species worldwide and is the cause of enzootic abortion of ewes (EAE). In Europe, control of the disease is achieved using a live vaccine based on C. abortus 1B strain. Although the vaccine has been useful for controlling disease outbreaks, abortion events due to the vaccine have been reported. Recently, placental pathology resulting from a vaccine type strain (vt) infection has been reported and shown to be similar to that resulting from a natural wild-type (wt) infection. The aim of this study was to extend these observations by comparing the distribution and severity of the lesions, the composition of the predominating cell infiltrate, the amount of bacteria present and the role of the blood supply in infection. A novel system for grading the histological and pathological features present was developed and the resulting multi-parameter data were statistically transformed for exploration and visualisation through a tailored principal component analysis (PCA) to evaluate the difference between them. The analysis provided no evidence of meaningful differences between vt and wt strains in terms of the measured pathological parameters. The study also contributes a novel methodology for analysing the progression of infection in the placenta for other abortifacient pathogens.
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spelling INTA95612021-06-11T12:06:52Z Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes Caspe, Sergio Gaston Palarea-Albaladejo, Javier Underwood, Clare Livingstone, Morag Wattegedera, Sean Ranjan Milne, Elspeth Sargison, Neil Donald Chianini, Francesca Longbottom, David Oveja Enfermedades de los Animales Chlamydophila abortus Vacuna Placenta Chlamydia Control de Enfermedades Ewes Animal Diseases Vaccines Diseases Control Chlamydia abortus Chlamydia abortus infects livestock species worldwide and is the cause of enzootic abortion of ewes (EAE). In Europe, control of the disease is achieved using a live vaccine based on C. abortus 1B strain. Although the vaccine has been useful for controlling disease outbreaks, abortion events due to the vaccine have been reported. Recently, placental pathology resulting from a vaccine type strain (vt) infection has been reported and shown to be similar to that resulting from a natural wild-type (wt) infection. The aim of this study was to extend these observations by comparing the distribution and severity of the lesions, the composition of the predominating cell infiltrate, the amount of bacteria present and the role of the blood supply in infection. A novel system for grading the histological and pathological features present was developed and the resulting multi-parameter data were statistically transformed for exploration and visualisation through a tailored principal component analysis (PCA) to evaluate the difference between them. The analysis provided no evidence of meaningful differences between vt and wt strains in terms of the measured pathological parameters. The study also contributes a novel methodology for analysing the progression of infection in the placenta for other abortifacient pathogens. EEA Mercedes Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido Fil: Caspe, Sergio Gaston. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: Underwood, Clare. Moredun Research Institute; Reino Unido Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Longbottom, David. Moredun Research Institute; Reino Unido 2021-06-11T11:59:10Z 2021-06-11T11:59:10Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9561 https://www.mdpi.com/2076-0817/10/5/543 2076-0817 https://doi.org/10.3390/pathogens10050543 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 10 (5) : 543 (April 2021)
spellingShingle Oveja
Enfermedades de los Animales
Chlamydophila abortus
Vacuna
Placenta
Chlamydia
Control de Enfermedades
Ewes
Animal Diseases
Vaccines
Diseases Control
Chlamydia abortus
Caspe, Sergio Gaston
Palarea-Albaladejo, Javier
Underwood, Clare
Livingstone, Morag
Wattegedera, Sean Ranjan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title_full Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title_fullStr Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title_full_unstemmed Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title_short Distribution and Severity of Placental Lesions Caused by the Chlamydia abortus 1B Vaccine Strain in Vaccinated Ewes
title_sort distribution and severity of placental lesions caused by the chlamydia abortus 1b vaccine strain in vaccinated ewes
topic Oveja
Enfermedades de los Animales
Chlamydophila abortus
Vacuna
Placenta
Chlamydia
Control de Enfermedades
Ewes
Animal Diseases
Vaccines
Diseases Control
Chlamydia abortus
url http://hdl.handle.net/20.500.12123/9561
https://www.mdpi.com/2076-0817/10/5/543
https://doi.org/10.3390/pathogens10050543
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