Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/956 http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa https://doi.org/10.1016/j.rvsc.2016.10.006 |
| _version_ | 1855482814885003264 |
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| author | Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio |
| author_browse | Aranaz, Alicia Canal, Ana María Cataldi, Angel Adrian Dominguez, Lucas Fernández, Analía Garbaccio, Sergio Gabriel Larzabal, Mariano Pezzone, Natalia Rodriguez-Bertos, Antonio Zumarraga, Martin Jose |
| author_facet | Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio |
| author_sort | Canal, Ana María |
| collection | INTA Digital |
| description | Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory
cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed.
Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma,
with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery
We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection.
High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas. |
| format | Artículo |
| id | INTA956 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9562019-03-22T12:52:17Z Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas. Inst. de Biotecnología Fil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina Fil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina Fil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España Fil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España Fil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España 2017-08-11T14:13:26Z 2017-08-11T14:13:26Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/956 http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa 0034-5288 https://doi.org/10.1016/j.rvsc.2016.10.006 eng info:eu-repo/semantics/restrictedAccess application/pdf Research in veterinary science 110 : 34-39. (2017) |
| spellingShingle | Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections Canal, Ana María Pezzone, Natalia Cataldi, Angel Adrian Zumarraga, Martin Jose Larzabal, Mariano Garbaccio, Sergio Gabriel Fernández, Analía Dominguez, Lucas Aranaz, Alicia Rodriguez-Bertos, Antonio Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title | Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title_full | Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title_fullStr | Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title_full_unstemmed | Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title_short | Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection |
| title_sort | immunohistochemical detection of pro inflammatory and anti inflammatory cytokines in granulomas in cattle with natural mycobacterium bovis infection |
| topic | Enfermedades de los Animales Mycobacterium bovis Ganado Bovino Citoquinas Infección por Mycobacterium bovis Animal Diseases Cattle Cytokines Mycobacterium bovis infections |
| url | http://hdl.handle.net/20.500.12123/956 http://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa https://doi.org/10.1016/j.rvsc.2016.10.006 |
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