Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection

Knowledge of linkage disequilibrium (LD) patterns is considered a prerequisite for effective association mapping studies. However, no LD analysis in the Argentine public temperate maize collection has been reported to date. In this study, a panel of 111 temperate maize inbreds genotyped at 74 single...

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Autores principales: Olmos, Sofía Eugenia, Lía, Verónica Viviana, Eyherabide, Guillermo
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/955
https://www.cambridge.org/core/journals/plant-genetic-resources/article/genetic-diversity-and-linkage-disequilibrium-in-the-argentine-public-maize-inbred-line-collection/A2B509D9C6A37824E41B40F6D92805D8
https://doi.org/10.1017/S1479262116000228
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author Olmos, Sofía Eugenia
Lía, Verónica Viviana
Eyherabide, Guillermo
author_browse Eyherabide, Guillermo
Lía, Verónica Viviana
Olmos, Sofía Eugenia
author_facet Olmos, Sofía Eugenia
Lía, Verónica Viviana
Eyherabide, Guillermo
author_sort Olmos, Sofía Eugenia
collection INTA Digital
description Knowledge of linkage disequilibrium (LD) patterns is considered a prerequisite for effective association mapping studies. However, no LD analysis in the Argentine public temperate maize collection has been reported to date. In this study, a panel of 111 temperate maize inbreds genotyped at 74 single sequence repeats (SSRs) loci was used to assess LD, genetic diversity and population structure to evaluate the suitability of the panel for association mapping. Mini-core sets were also designed for in-depth phenotyping and allele mining purposes. The panel consisted of: (1) locally developed orange flint germplasm; (2) temperate inbred lines with Iowa Stiff Stalk Synthetic background; and (3) eight historic flint lines, some of them from the Cuarentín race. As a result, four subpopulations were defined. Joint analysis of population structure and combining ability allowed identifying two main heterotic patterns. High molecular diversity, a low extent of LD and a high ratio of linked to unlinked SSR loci pairs in significant LD were detected indicating the suitability of the entire collection for association mapping. The fact that the LD extent in the mini-core sets was similar to that observed in the entire collection and that only a small percentage of allelic richness was reduced suggests that these mini-core sets are suitable to capture diversity, exploit phenotypic variance and discover useful variants representative of the entire collection.
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spelling INTA9552019-03-22T12:51:09Z Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection Olmos, Sofía Eugenia Lía, Verónica Viviana Eyherabide, Guillermo Genética Maíz Variación Genética Lineas Consanguíneas Genetics Maize Genetic Variation Inbred Lines Diversidad Genética Knowledge of linkage disequilibrium (LD) patterns is considered a prerequisite for effective association mapping studies. However, no LD analysis in the Argentine public temperate maize collection has been reported to date. In this study, a panel of 111 temperate maize inbreds genotyped at 74 single sequence repeats (SSRs) loci was used to assess LD, genetic diversity and population structure to evaluate the suitability of the panel for association mapping. Mini-core sets were also designed for in-depth phenotyping and allele mining purposes. The panel consisted of: (1) locally developed orange flint germplasm; (2) temperate inbred lines with Iowa Stiff Stalk Synthetic background; and (3) eight historic flint lines, some of them from the Cuarentín race. As a result, four subpopulations were defined. Joint analysis of population structure and combining ability allowed identifying two main heterotic patterns. High molecular diversity, a low extent of LD and a high ratio of linked to unlinked SSR loci pairs in significant LD were detected indicating the suitability of the entire collection for association mapping. The fact that the LD extent in the mini-core sets was similar to that observed in the entire collection and that only a small percentage of allelic richness was reduced suggests that these mini-core sets are suitable to capture diversity, exploit phenotypic variance and discover useful variants representative of the entire collection. Inst. de Biotecnología Fil: Olmos, Sofí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Eyherabide, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina 2017-08-11T13:34:18Z 2017-08-11T13:34:18Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/955 https://www.cambridge.org/core/journals/plant-genetic-resources/article/genetic-diversity-and-linkage-disequilibrium-in-the-argentine-public-maize-inbred-line-collection/A2B509D9C6A37824E41B40F6D92805D8 1479-2621 (Print) 1479-263X (Online) https://doi.org/10.1017/S1479262116000228 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Plant genetic resources 5 (6) : 515-526. (December 2017)
spellingShingle Genética
Maíz
Variación Genética
Lineas Consanguíneas
Genetics
Maize
Genetic Variation
Inbred Lines
Diversidad Genética
Olmos, Sofía Eugenia
Lía, Verónica Viviana
Eyherabide, Guillermo
Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title_full Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title_fullStr Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title_full_unstemmed Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title_short Genetic diversity and linkage disequilibrium in the Argentine public maize inbred line collection
title_sort genetic diversity and linkage disequilibrium in the argentine public maize inbred line collection
topic Genética
Maíz
Variación Genética
Lineas Consanguíneas
Genetics
Maize
Genetic Variation
Inbred Lines
Diversidad Genética
url http://hdl.handle.net/20.500.12123/955
https://www.cambridge.org/core/journals/plant-genetic-resources/article/genetic-diversity-and-linkage-disequilibrium-in-the-argentine-public-maize-inbred-line-collection/A2B509D9C6A37824E41B40F6D92805D8
https://doi.org/10.1017/S1479262116000228
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