Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay
Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified inte...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/954 http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub https://doi.org/10.1016/j.virusres.2017.01.002 |
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| author | Llauger, Gabriela De Haro, Luis Alejandro Alfonso, Victoria Del Vas, Mariana |
| author_browse | Alfonso, Victoria De Haro, Luis Alejandro Del Vas, Mariana Llauger, Gabriela |
| author_facet | Llauger, Gabriela De Haro, Luis Alejandro Alfonso, Victoria Del Vas, Mariana |
| author_sort | Llauger, Gabriela |
| collection | INTA Digital |
| description | Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified interactionsof non-structural proteins P6/P6, P9-2/P9-2 and P6/P9-1. P9-1 and P6 are the major and minor compo-nents of the viroplasms respectively, whereas P9-2 is an N-glycosylated membrane protein of unknownfunction. Interactions involving P6 and P9-1 were confirmed by bimolecular fluorescence complementa-tion (BiFC) in rice protoplasts. We demonstrated that a region including a predicted coiled-coil domainwithin the C-terminal moiety of P6 was necessary for P6/P6 and P6/P9-1 interactions. In turn, a shortC-terminal arm was necessary for the previously reported P9-1 self-interaction. Transient expression ofthese proteins by agroinfiltration of Nicotiana benthamiana leaves showed very low accumulation levelsand further in silico analyses allowed us to identify conserved PEST degradation sequences [rich in pro-line (P), glutamic acid (E), serine (S), and threonine (T)] within P6 and P9-1. The removal of these PESTsequences resulted in a significant increase of the accumulation of both proteins. |
| format | info:ar-repo/semantics/artículo |
| id | INTA954 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9542019-03-22T12:49:30Z Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay Llauger, Gabriela De Haro, Luis Alejandro Alfonso, Victoria Del Vas, Mariana Fitomejoramiento Enfermedades de las Plantas Virus de las Plantas Identificación Plant Breeding Plant Diseases Plant viruses Identification Mal de Río Cuarto Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified interactionsof non-structural proteins P6/P6, P9-2/P9-2 and P6/P9-1. P9-1 and P6 are the major and minor compo-nents of the viroplasms respectively, whereas P9-2 is an N-glycosylated membrane protein of unknownfunction. Interactions involving P6 and P9-1 were confirmed by bimolecular fluorescence complementa-tion (BiFC) in rice protoplasts. We demonstrated that a region including a predicted coiled-coil domainwithin the C-terminal moiety of P6 was necessary for P6/P6 and P6/P9-1 interactions. In turn, a shortC-terminal arm was necessary for the previously reported P9-1 self-interaction. Transient expression ofthese proteins by agroinfiltration of Nicotiana benthamiana leaves showed very low accumulation levelsand further in silico analyses allowed us to identify conserved PEST degradation sequences [rich in pro-line (P), glutamic acid (E), serine (S), and threonine (T)] within P6 and P9-1. The removal of these PESTsequences resulted in a significant increase of the accumulation of both proteins. Inst. de Biotecnología Fil: Llauger, GabrielaInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-08-11T13:05:10Z 2017-08-11T13:05:10Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/954 http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub 0168-1702 https://doi.org/10.1016/j.virusres.2017.01.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Virus research 230 :19-28. (2017) |
| spellingShingle | Fitomejoramiento Enfermedades de las Plantas Virus de las Plantas Identificación Plant Breeding Plant Diseases Plant viruses Identification Mal de Río Cuarto Llauger, Gabriela De Haro, Luis Alejandro Alfonso, Victoria Del Vas, Mariana Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title_full | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title_fullStr | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title_full_unstemmed | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title_short | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay |
| title_sort | interaction of mal de rio cuarto virus fijivirus genus proteins and identification of putative factors determining viroplasm formation and decay |
| topic | Fitomejoramiento Enfermedades de las Plantas Virus de las Plantas Identificación Plant Breeding Plant Diseases Plant viruses Identification Mal de Río Cuarto |
| url | http://hdl.handle.net/20.500.12123/954 http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub https://doi.org/10.1016/j.virusres.2017.01.002 |
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