Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense
Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR al...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9537 https://www.mdpi.com/2073-4395/11/2/195 https://doi.org/10.3390/agronomy11020195 |
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| author | Viejobueno, Josefina Albornoz, Patricia Liliana Camacho, María Santos, Berta de los Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel |
| author_browse | Albornoz, Patricia Liliana Camacho, María Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Santos, Berta de los Viejobueno, Josefina |
| author_facet | Viejobueno, Josefina Albornoz, Patricia Liliana Camacho, María Santos, Berta de los Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel |
| author_sort | Viejobueno, Josefina |
| collection | INTA Digital |
| description | Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way. |
| format | Artículo |
| id | INTA9537 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA95372021-06-09T13:36:02Z Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense Viejobueno, Josefina Albornoz, Patricia Liliana Camacho, María Santos, Berta de los Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Fresa Protección de las Plantas Macrophomina phaseolina Azospirillum brasilense Sustancias de Crecimiento Vegetal Rhizobacteria Strawberries Crop Protection Plant Growth Substances Frutilla Charcoal Rot Disease Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way. EEA Famaillá Fil: Viejobueno, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Albornoz, Patricia Liliana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina Fil: Albornoz, Patricia Liliana. Fundación Miguel Lillo; Argentina Fil: Camacho, María. Instituto de Investigación y Formación Agraria y Pesquera; España Fil: Santos, Berta de los. Instituto de Investigación y Formación Agraria y Pesquera; España Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina 2021-06-09T13:28:46Z 2021-06-09T13:28:46Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9537 https://www.mdpi.com/2073-4395/11/2/195 2073-4395 https://doi.org/10.3390/agronomy11020195 eng info:eu-repograntAgreement/INTA/PNHFA-1106073/AR./Aumento de la competitividad con sustentabilidad y equidad social de sistemas productivos de hortalizas frescas diferenciadas. info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Agronomy 11 (2) : 195 (2021) |
| spellingShingle | Fresa Protección de las Plantas Macrophomina phaseolina Azospirillum brasilense Sustancias de Crecimiento Vegetal Rhizobacteria Strawberries Crop Protection Plant Growth Substances Frutilla Charcoal Rot Disease Viejobueno, Josefina Albornoz, Patricia Liliana Camacho, María Santos, Berta de los Martinez Zamora, Martin Gustavo Salazar, Sergio Miguel Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title | Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title_full | Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title_fullStr | Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title_full_unstemmed | Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title_short | Protection of Strawberry Plants against Charcoal Rot Disease (Macrophomina phaseolina) Induced by Azospirillum brasilense |
| title_sort | protection of strawberry plants against charcoal rot disease macrophomina phaseolina induced by azospirillum brasilense |
| topic | Fresa Protección de las Plantas Macrophomina phaseolina Azospirillum brasilense Sustancias de Crecimiento Vegetal Rhizobacteria Strawberries Crop Protection Plant Growth Substances Frutilla Charcoal Rot Disease |
| url | http://hdl.handle.net/20.500.12123/9537 https://www.mdpi.com/2073-4395/11/2/195 https://doi.org/10.3390/agronomy11020195 |
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