In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice
The objective of this work was to determine the antibacterial effect of Lactobacillus plantarum strains of pork origin against Campylobacter coli strains, and to conduct experimental colonization pilot models in mice for both microorganisms. Inhibition assays allowed evaluation and selection of L. p...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/9490 https://link.springer.com/article/10.1007/s00203-021-02385-5 https://doi.org/10.1007/s00203-021-02385-5 |
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| author | Ruiz, María Julia Zbrun, María Virginia Signorini Porchiett, Marcelo Lisandro Zimmermann, Jorge Alberto Soto, Lorena Paola Rosmini, Marcelo Raúl Frizzo, Laureano Sebastian |
| author_browse | Frizzo, Laureano Sebastian Rosmini, Marcelo Raúl Ruiz, María Julia Signorini Porchiett, Marcelo Lisandro Soto, Lorena Paola Zbrun, María Virginia Zimmermann, Jorge Alberto |
| author_facet | Ruiz, María Julia Zbrun, María Virginia Signorini Porchiett, Marcelo Lisandro Zimmermann, Jorge Alberto Soto, Lorena Paola Rosmini, Marcelo Raúl Frizzo, Laureano Sebastian |
| author_sort | Ruiz, María Julia |
| collection | INTA Digital |
| description | The objective of this work was to determine the antibacterial effect of Lactobacillus plantarum strains of pork origin against Campylobacter coli strains, and to conduct experimental colonization pilot models in mice for both microorganisms. Inhibition assays allowed evaluation and selection of L. plantarum LP5 as the strain with the highest antagonistic activity against C. coli and with the best potential to be used in in vivo study. Adult 6-week-old female Balb/cCmedc mice were lodged in two groups. The treated group was administered with 9.4 log10CFU/2 times/wk of L. plantarum LP5. L. plantarum LP5 was recovered from the feces and cecum of the inoculated mice. However, when bacteria stopped being administered, probiotic counts decreased. Experimental colonization with C. coli was carried out in five groups of mice. All animals were treated with antibiotics in their drinking water to weaken the indigenous microbiota and to allow colonization of C. coli. Four groups were administered once with different C. coli strains (DSPV458: 8.49 log10CFU; DSPV567: 8.09 log10CFU; DSPV570: 8.46 log10CFU; DSPV541: 8.86 log10CFU, respectively). After 8 h, mice inoculated with different C. coli strains were colonized because the pathogen was detected in their feces. L. plantarum LP5 tolerated the gastrointestinal conditions of murine model without generating adverse effects on the animals. C. coli DSPV458 colonized the mice without causing infection by lodging in their digestive tract, thus generating a reproducible colonization model. Both models combined could be used as protection murine models against pathogens to test alternative control tools to antibiotics. |
| format | Artículo |
| id | INTA9490 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA94902022-06-16T13:14:58Z In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice Ruiz, María Julia Zbrun, María Virginia Signorini Porchiett, Marcelo Lisandro Zimmermann, Jorge Alberto Soto, Lorena Paola Rosmini, Marcelo Raúl Frizzo, Laureano Sebastian Lactobacillus plantarum Campylobacter coli Experimentación in Vitro Experimentación in Vivo Ratón In Vitro Experimentation In Vivo Experimentation Mice The objective of this work was to determine the antibacterial effect of Lactobacillus plantarum strains of pork origin against Campylobacter coli strains, and to conduct experimental colonization pilot models in mice for both microorganisms. Inhibition assays allowed evaluation and selection of L. plantarum LP5 as the strain with the highest antagonistic activity against C. coli and with the best potential to be used in in vivo study. Adult 6-week-old female Balb/cCmedc mice were lodged in two groups. The treated group was administered with 9.4 log10CFU/2 times/wk of L. plantarum LP5. L. plantarum LP5 was recovered from the feces and cecum of the inoculated mice. However, when bacteria stopped being administered, probiotic counts decreased. Experimental colonization with C. coli was carried out in five groups of mice. All animals were treated with antibiotics in their drinking water to weaken the indigenous microbiota and to allow colonization of C. coli. Four groups were administered once with different C. coli strains (DSPV458: 8.49 log10CFU; DSPV567: 8.09 log10CFU; DSPV570: 8.46 log10CFU; DSPV541: 8.86 log10CFU, respectively). After 8 h, mice inoculated with different C. coli strains were colonized because the pathogen was detected in their feces. L. plantarum LP5 tolerated the gastrointestinal conditions of murine model without generating adverse effects on the animals. C. coli DSPV458 colonized the mice without causing infection by lodging in their digestive tract, thus generating a reproducible colonization model. Both models combined could be used as protection murine models against pathogens to test alternative control tools to antibiotics. EEA Rafaela Fil: Ruiz, M.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ruiz, M.J. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ruiz, M.J. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina; Argentina Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Rosmini, Marcelo Raúl. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina 2021-06-03T14:38:48Z 2021-06-03T14:38:48Z 2021-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9490 https://link.springer.com/article/10.1007/s00203-021-02385-5 0302-8933 1432-072X https://doi.org/10.1007/s00203-021-02385-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Archives of Microbiology (Published: 01 June 2021) |
| spellingShingle | Lactobacillus plantarum Campylobacter coli Experimentación in Vitro Experimentación in Vivo Ratón In Vitro Experimentation In Vivo Experimentation Mice Ruiz, María Julia Zbrun, María Virginia Signorini Porchiett, Marcelo Lisandro Zimmermann, Jorge Alberto Soto, Lorena Paola Rosmini, Marcelo Raúl Frizzo, Laureano Sebastian In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title | In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title_full | In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title_fullStr | In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title_full_unstemmed | In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title_short | In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice |
| title_sort | in vitro screening and in vivo colonization pilot model of lactobacillus plantarum lp5 and campylobacter coli dspv 458 in mice |
| topic | Lactobacillus plantarum Campylobacter coli Experimentación in Vitro Experimentación in Vivo Ratón In Vitro Experimentation In Vivo Experimentation Mice |
| url | http://hdl.handle.net/20.500.12123/9490 https://link.springer.com/article/10.1007/s00203-021-02385-5 https://doi.org/10.1007/s00203-021-02385-5 |
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