Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments:...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9489 https://onlinelibrary.wiley.com/doi/10.1002/jobm.202100064 https://doi.org/10.1002/jobm.202100064 |
| _version_ | 1855036222857019392 |
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| author | Toffoli, Lucia Mercedes Martinez Zamora, Martin Gustavo Medrano, Norma Nelly Fontana, Cecilia Alejandra Lovaisa, Nadia Carolina Delaporte Quintana, Paola Adriana Georgina Elias, Juliana M. Salazar, Sergio Miguel Pedraza, Raúl Osvaldo |
| author_browse | Delaporte Quintana, Paola Adriana Georgina Elias, Juliana M. Fontana, Cecilia Alejandra Lovaisa, Nadia Carolina Martinez Zamora, Martin Gustavo Medrano, Norma Nelly Pedraza, Raúl Osvaldo Salazar, Sergio Miguel Toffoli, Lucia Mercedes |
| author_facet | Toffoli, Lucia Mercedes Martinez Zamora, Martin Gustavo Medrano, Norma Nelly Fontana, Cecilia Alejandra Lovaisa, Nadia Carolina Delaporte Quintana, Paola Adriana Georgina Elias, Juliana M. Salazar, Sergio Miguel Pedraza, Raúl Osvaldo |
| author_sort | Toffoli, Lucia Mercedes |
| collection | INTA Digital |
| description | To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9489 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA94892023-07-28T14:11:46Z Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering Toffoli, Lucia Mercedes Martinez Zamora, Martin Gustavo Medrano, Norma Nelly Fontana, Cecilia Alejandra Lovaisa, Nadia Carolina Delaporte Quintana, Paola Adriana Georgina Elias, Juliana M. Salazar, Sergio Miguel Pedraza, Raúl Osvaldo Petunia Floración Azospirillum brasilense Sideróforos Índoles Flowering Siderophores Indoles Plant Growth Substances Sustancias de Crecimiento Vegetal To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering. EEA Famaillá Fil: Toffoli, Lucia Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Medrano, Norma Nelly. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Fil: Delaporte Quintana, Paola Adriana Georgina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Elías, J. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina 2021-06-03T13:34:47Z 2021-06-03T13:34:47Z 2021-05-31 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9489 https://onlinelibrary.wiley.com/doi/10.1002/jobm.202100064 0233-111X 1521-4028 https://doi.org/10.1002/jobm.202100064 eng info:eu-repograntAgreement/INTA/PNHFA-1106093/AR./Desarrollo y ajuste de tecnologías para una producción florícola sustentable y de calidad. info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Journal of Basic Microbiology 61 (7) : 662-673. (July 2021) |
| spellingShingle | Petunia Floración Azospirillum brasilense Sideróforos Índoles Flowering Siderophores Indoles Plant Growth Substances Sustancias de Crecimiento Vegetal Toffoli, Lucia Mercedes Martinez Zamora, Martin Gustavo Medrano, Norma Nelly Fontana, Cecilia Alejandra Lovaisa, Nadia Carolina Delaporte Quintana, Paola Adriana Georgina Elias, Juliana M. Salazar, Sergio Miguel Pedraza, Raúl Osvaldo Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title | Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title_full | Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title_fullStr | Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title_full_unstemmed | Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title_short | Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| title_sort | natural occurrence of azospirillum brasilense in petunia with capacity to improve plant growth and flowering |
| topic | Petunia Floración Azospirillum brasilense Sideróforos Índoles Flowering Siderophores Indoles Plant Growth Substances Sustancias de Crecimiento Vegetal |
| url | http://hdl.handle.net/20.500.12123/9489 https://onlinelibrary.wiley.com/doi/10.1002/jobm.202100064 https://doi.org/10.1002/jobm.202100064 |
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