Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-...
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| Format: | Artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/947 https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962 https://doi.org/10.1017/S0950268817000139 |
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| author | Imperiale, Belen Rocio Moyano, Roberto Damian Di Giulio, A.B. Romero, M.A. Alvarado Pinedo, Maria Fiorella Santangelo, María De La Paz Travería, G.E. Morcillo, N.S. Romano, Maria Isabel |
| author_browse | Alvarado Pinedo, Maria Fiorella Di Giulio, A.B. Imperiale, Belen Rocio Morcillo, N.S. Moyano, Roberto Damian Romano, Maria Isabel Romero, M.A. Santangelo, María De La Paz Travería, G.E. |
| author_facet | Imperiale, Belen Rocio Moyano, Roberto Damian Di Giulio, A.B. Romero, M.A. Alvarado Pinedo, Maria Fiorella Santangelo, María De La Paz Travería, G.E. Morcillo, N.S. Romano, Maria Isabel |
| author_sort | Imperiale, Belen Rocio |
| collection | INTA Digital |
| description | Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The
IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP.
The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples. |
| format | Artículo |
| id | INTA947 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9472019-03-22T12:45:58Z Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR Imperiale, Belen Rocio Moyano, Roberto Damian Di Giulio, A.B. Romero, M.A. Alvarado Pinedo, Maria Fiorella Santangelo, María De La Paz Travería, G.E. Morcillo, N.S. Romano, Maria Isabel Genética Mycobacterium avium Variación Genética Genetics Genetic Variation Diversidad Genética Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples. Inst. de Biotecnología Fil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Giulio, A.B. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; San Fernando, Argentina Fil: Romero, M.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Travería, G.E. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina Fil: Morcillo, Nora S.Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-08-10T15:48:13Z 2017-08-10T15:48:13Z 2017-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/947 https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962 0950-2688 (Print) 1469-4409 (Online) https://doi.org/10.1017/S0950268817000139 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Epidemiology and infection 145 (7) : 1382-1391. (2017) |
| spellingShingle | Genética Mycobacterium avium Variación Genética Genetics Genetic Variation Diversidad Genética Imperiale, Belen Rocio Moyano, Roberto Damian Di Giulio, A.B. Romero, M.A. Alvarado Pinedo, Maria Fiorella Santangelo, María De La Paz Travería, G.E. Morcillo, N.S. Romano, Maria Isabel Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title | Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title_full | Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title_fullStr | Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title_full_unstemmed | Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title_short | Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR |
| title_sort | genetic diversity of mycobacterium avium complex strains isolated in argentina by miru vntr |
| topic | Genética Mycobacterium avium Variación Genética Genetics Genetic Variation Diversidad Genética |
| url | http://hdl.handle.net/20.500.12123/947 https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962 https://doi.org/10.1017/S0950268817000139 |
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