Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus
MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at posttranscriptional level and play essential roles in biological processes such as development. MiRNAs silence target mRNAs by binding to complementary sequences in the 3′untranslated regions (3′UTRs). The...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/945 http://www.sciencedirect.com/science/article/pii/S0166685117300488?via%3Dihub https://doi.org/10.1016/j.molbiopara.2017.04.001 |
| _version_ | 1855482812890611712 |
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| author | Macchiaroli, Natalia Maldonado, Lucas Zarowiecki, Magdalena Cucher, Marcela Alejandra Gismondi, Maria Ines Kamenetzky, Laura Rosenzvit, Mara Cecilia |
| author_browse | Cucher, Marcela Alejandra Gismondi, Maria Ines Kamenetzky, Laura Macchiaroli, Natalia Maldonado, Lucas Rosenzvit, Mara Cecilia Zarowiecki, Magdalena |
| author_facet | Macchiaroli, Natalia Maldonado, Lucas Zarowiecki, Magdalena Cucher, Marcela Alejandra Gismondi, Maria Ines Kamenetzky, Laura Rosenzvit, Mara Cecilia |
| author_sort | Macchiaroli, Natalia |
| collection | INTA Digital |
| description | MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at posttranscriptional level and play essential roles in biological processes such as development. MiRNAs silence target mRNAs by binding to complementary sequences in the 3′untranslated regions (3′UTRs). The parasitic helminths of the genus Echinococcus are the causative agents of echinococcosis, a zoonotic neglected disease. In previous work, we performed a comprehensive identification and characterization of Echinococcus miRNAs. However, current knowledge about their targets is limited. Since target prediction algorithms rely on complementarity between 3′UTRs and miRNA sequences, a major limitation is the lack of accurate sequence information of 3′UTR for most species including parasitic helminths. We performed RNA-seq and developed a pipeline that integrates the transcriptomic data with available genomic data of this parasite in order to identify 3′UTRs of Echinococcus canadensis. The high confidence set of 3′UTRs obtained allowed the prediction of miRNA targets in Echinococcus through a bioinformatic approach. We performed for the first time a comparative analysis of miRNA targets in Echinococcus and Taenia. We found that many evolutionarily conserved target sites in Echinococcus and Taenia may be functional and under selective pressure. Signaling pathways such as MAPK and Wnt were among the
most represented pathways indicating miRNA roles in parasite growth and development. Genome-wide identification and characterization of miRNA target genes in Echinococcus provide valuable information to guide experimental studies in order to understand miRNA functions in the parasites biology. miRNAs involved in essential functions, especially those being absent in the host or showing sequence divergence with respect to host orthologs, might be considered as novel therapeutic targets for echinococcosis control. |
| format | Artículo |
| id | INTA945 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9452019-03-22T12:42:17Z Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus Macchiaroli, Natalia Maldonado, Lucas Zarowiecki, Magdalena Cucher, Marcela Alejandra Gismondi, Maria Ines Kamenetzky, Laura Rosenzvit, Mara Cecilia Genética ARN Echinococcus Patógenos RNA Genetics Pathogens MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at posttranscriptional level and play essential roles in biological processes such as development. MiRNAs silence target mRNAs by binding to complementary sequences in the 3′untranslated regions (3′UTRs). The parasitic helminths of the genus Echinococcus are the causative agents of echinococcosis, a zoonotic neglected disease. In previous work, we performed a comprehensive identification and characterization of Echinococcus miRNAs. However, current knowledge about their targets is limited. Since target prediction algorithms rely on complementarity between 3′UTRs and miRNA sequences, a major limitation is the lack of accurate sequence information of 3′UTR for most species including parasitic helminths. We performed RNA-seq and developed a pipeline that integrates the transcriptomic data with available genomic data of this parasite in order to identify 3′UTRs of Echinococcus canadensis. The high confidence set of 3′UTRs obtained allowed the prediction of miRNA targets in Echinococcus through a bioinformatic approach. We performed for the first time a comparative analysis of miRNA targets in Echinococcus and Taenia. We found that many evolutionarily conserved target sites in Echinococcus and Taenia may be functional and under selective pressure. Signaling pathways such as MAPK and Wnt were among the most represented pathways indicating miRNA roles in parasite growth and development. Genome-wide identification and characterization of miRNA target genes in Echinococcus provide valuable information to guide experimental studies in order to understand miRNA functions in the parasites biology. miRNAs involved in essential functions, especially those being absent in the host or showing sequence divergence with respect to host orthologs, might be considered as novel therapeutic targets for echinococcosis control. Inst. de Biotecnología Fil: Macchiaroli, Natalia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maldonado, Lucas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zarowiecki, Magdalena. Wellcome Trust Sanger Institute. Parasite Genomics Group; Reino Unido Fil: Cucher, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médicas; Argentina Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rosenzvit, Mara Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médicas; Argentina 2017-08-10T13:48:27Z 2017-08-10T13:48:27Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/945 http://www.sciencedirect.com/science/article/pii/S0166685117300488?via%3Dihub 0166-6851 https://doi.org/10.1016/j.molbiopara.2017.04.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Molecular and biochemical parasitology 214 : 91-100. (2017) |
| spellingShingle | Genética ARN Echinococcus Patógenos RNA Genetics Pathogens Macchiaroli, Natalia Maldonado, Lucas Zarowiecki, Magdalena Cucher, Marcela Alejandra Gismondi, Maria Ines Kamenetzky, Laura Rosenzvit, Mara Cecilia Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title | Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title_full | Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title_fullStr | Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title_full_unstemmed | Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title_short | Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus |
| title_sort | genome wide identification of microrna targets in the neglected disease pathogens of the genus echinococcus |
| topic | Genética ARN Echinococcus Patógenos RNA Genetics Pathogens |
| url | http://hdl.handle.net/20.500.12123/945 http://www.sciencedirect.com/science/article/pii/S0166685117300488?via%3Dihub https://doi.org/10.1016/j.molbiopara.2017.04.001 |
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