Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)

The study of spermatogenesis in Hymenoptera is limited to few taxa due to limitations to access the material in the right stage for analysis. The information available up to now states that the gametogenesis in males involves a modified meiosis, similar to mitosis. This is due to the haplodiploid se...

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Autores principales: Carabajal Paladino, Leonale Suzel, Lo Nostro, Fabiana Laura, Papeschi, Alba Graciela, Cladera, Jorge Luis, Bressa, María José
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/943
http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/abstract
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author Carabajal Paladino, Leonale Suzel
Lo Nostro, Fabiana Laura
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
author_browse Bressa, María José
Carabajal Paladino, Leonale Suzel
Cladera, Jorge Luis
Lo Nostro, Fabiana Laura
Papeschi, Alba Graciela
author_facet Carabajal Paladino, Leonale Suzel
Lo Nostro, Fabiana Laura
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
author_sort Carabajal Paladino, Leonale Suzel
collection INTA Digital
description The study of spermatogenesis in Hymenoptera is limited to few taxa due to limitations to access the material in the right stage for analysis. The information available up to now states that the gametogenesis in males involves a modified meiosis, similar to mitosis. This is due to the haplodiploid sex determination system present in the order, where females are diploid and males are haploid. In the present work, we examined the spermatogenesis process in the parasitoid Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). We performed squash preparations from testes of individuals from the third larval instar until the adult stage. Some cytological traits, such as the presence of a monopolar spindle, were analysed in detail by means of histological semi-thin sections. The spermatogenesis in this species comprises an abortive first division with the formation of an anucleated cytoplasmic bud and a nucleated cell, and an equal second division of the nucleated cell, leading to the generation of two mature spermatozoa. The results presented herein provide more information about the process of spermatogenesis in Hymenoptera, which has not been studied in depth in parasitic species.
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spelling INTA9432018-02-26T13:23:01Z Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae) Carabajal Paladino, Leonale Suzel Lo Nostro, Fabiana Laura Papeschi, Alba Graciela Cladera, Jorge Luis Bressa, María José Genética Biosteres longicaudatus Parasitoides Espermatogénesis Genetics Parasitoids Spermatogenesis Diachasmimorpha longicaudata The study of spermatogenesis in Hymenoptera is limited to few taxa due to limitations to access the material in the right stage for analysis. The information available up to now states that the gametogenesis in males involves a modified meiosis, similar to mitosis. This is due to the haplodiploid sex determination system present in the order, where females are diploid and males are haploid. In the present work, we examined the spermatogenesis process in the parasitoid Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). We performed squash preparations from testes of individuals from the third larval instar until the adult stage. Some cytological traits, such as the presence of a monopolar spindle, were analysed in detail by means of histological semi-thin sections. The spermatogenesis in this species comprises an abortive first division with the formation of an anucleated cytoplasmic bud and a nucleated cell, and an equal second division of the nucleated cell, leading to the generation of two mature spermatozoa. The results presented herein provide more information about the process of spermatogenesis in Hymenoptera, which has not been studied in depth in parasitic species. Inst. de Genética "Ewald A. Favret"- IGEAF Fil: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina 2017-08-10T12:25:01Z 2017-08-10T12:25:01Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/943 http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/abstract 1463-6395 DOI: 10.1111/azo.12148 eng info:eu-repo/semantics/restrictedAccess application/pdf Acta Zoologica 98 (1) : 38-43
spellingShingle Genética
Biosteres longicaudatus
Parasitoides
Espermatogénesis
Genetics
Parasitoids
Spermatogenesis
Diachasmimorpha longicaudata
Carabajal Paladino, Leonale Suzel
Lo Nostro, Fabiana Laura
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title_full Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title_fullStr Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title_full_unstemmed Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title_short Spermatogenesis in the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae)
title_sort spermatogenesis in the parasitoid diachasmimorpha longicaudata hymenoptera braconidae
topic Genética
Biosteres longicaudatus
Parasitoides
Espermatogénesis
Genetics
Parasitoids
Spermatogenesis
Diachasmimorpha longicaudata
url http://hdl.handle.net/20.500.12123/943
http://onlinelibrary.wiley.com/doi/10.1111/azo.12148/abstract
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