Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina
S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study w...
| Autores principales: | , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9422 https://link.springer.com/article/10.1007/s10658-021-02288-7 https://doi.org/10.1007/s10658-021-02288-7 |
| _version_ | 1855036210299273216 |
|---|---|
| author | Aban, Carla Luciana Taboada, Gisel María Spedaleti, Yamila Andrea Maita, Efraín Galvan, Marta Zulema |
| author_browse | Aban, Carla Luciana Galvan, Marta Zulema Maita, Efraín Spedaleti, Yamila Andrea Taboada, Gisel María |
| author_facet | Aban, Carla Luciana Taboada, Gisel María Spedaleti, Yamila Andrea Maita, Efraín Galvan, Marta Zulema |
| author_sort | Aban, Carla Luciana |
| collection | INTA Digital |
| description | S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9422 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA94222021-05-26T12:36:53Z Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina Aban, Carla Luciana Taboada, Gisel María Spedaleti, Yamila Andrea Maita, Efraín Galvan, Marta Zulema Fríjol (phaseolus) Sclerotinia sclerotiorum Hongos Estructura de la Población Argentina Kidney Beans Fungi Population Structure Common Beans S. sclerotiorum is the causal agent of the white mould disease on common bean crops, one of the most threatening fungal diseases occurring across major bean production regions. Currently, there are no commercial cultivars with high levels of genetic resistance to white mould. The aim of this study was to analyse the population structure of 109 isolates of S. sclerotiorum from six dry bean fields in the main production area of Argentina using nine microsatellite loci. A total of 30 multilocus haplotypes (MLHs) were identified, of which 18 MLHs were unique. The remaining 12 MLHs were constituted by 83% of the isolates, six MLHs of which (composed of 75 isolates) were shared at least between two locations. Population genetic structure analysis and discriminant analysis of principal components (DAPC) identified two genetic clusters (subpopulations). The genetic cluster 1 (GC 1) was composed of 21 isolates and 15 MLHs. Similarly, the genetic cluster 2 (GC 2) was composed of 23 isolates and 15 MLHs. These two genetic clusters were observed in most locations sampled. Low levels of genetic differentiation (ΦST = 0.198; P < 0.0001) followed by high levels of gene flow (Nm > 1) between genetic clusters were observed. Linkage disequilibrium analysis showed that one of the two genetic subpopulations was under linkage equilibrium (P > 0.001), which is consistent with recombinant populations. These results suggest the occurrence of both modes of reproductive behaviour, clonal and recombining, compromising the durability of management strategies for white mould disease in common bean cultivars. EEA Salta Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina Fil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina Fil: Spedaleti, Yamila Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Maita, Efraín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina Fil: Maita, Efraín Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Investigaciones Botánicas; Argentina Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina 2021-05-26T12:32:30Z 2021-05-26T12:32:30Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9422 https://link.springer.com/article/10.1007/s10658-021-02288-7 0929-1873 1573-8469 https://doi.org/10.1007/s10658-021-02288-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology (Published: 10 May 2021) |
| spellingShingle | Fríjol (phaseolus) Sclerotinia sclerotiorum Hongos Estructura de la Población Argentina Kidney Beans Fungi Population Structure Common Beans Aban, Carla Luciana Taboada, Gisel María Spedaleti, Yamila Andrea Maita, Efraín Galvan, Marta Zulema Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title | Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title_full | Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title_fullStr | Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title_full_unstemmed | Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title_short | Population structure of the fungus Sclerotinia sclerotiorum in common bean fields of Argentina |
| title_sort | population structure of the fungus sclerotinia sclerotiorum in common bean fields of argentina |
| topic | Fríjol (phaseolus) Sclerotinia sclerotiorum Hongos Estructura de la Población Argentina Kidney Beans Fungi Population Structure Common Beans |
| url | http://hdl.handle.net/20.500.12123/9422 https://link.springer.com/article/10.1007/s10658-021-02288-7 https://doi.org/10.1007/s10658-021-02288-7 |
| work_keys_str_mv | AT abancarlaluciana populationstructureofthefungussclerotiniasclerotiorumincommonbeanfieldsofargentina AT taboadagiselmaria populationstructureofthefungussclerotiniasclerotiorumincommonbeanfieldsofargentina AT spedaletiyamilaandrea populationstructureofthefungussclerotiniasclerotiorumincommonbeanfieldsofargentina AT maitaefrain populationstructureofthefungussclerotiniasclerotiorumincommonbeanfieldsofargentina AT galvanmartazulema populationstructureofthefungussclerotiniasclerotiorumincommonbeanfieldsofargentina |