Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9283 https://link.springer.com/article/10.1007/s11033-020-05554-9 https://doi.org/10.1007/s11033-020-05554-9 |
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| author | Contreras Valentín, Ana Cibeles Mariotti, Roberto Mousavi, S. Baldoni, L. Guerrero, C. Roka, L. Cultrera, N. Pierantozzi, Pierluigi Maestri, Damián Gentili, Luciana Tivani, Martín Torres, Myriam Mariela |
| author_browse | Baldoni, L. Contreras Valentín, Ana Cibeles Cultrera, N. Gentili, Luciana Guerrero, C. Maestri, Damián Mariotti, Roberto Mousavi, S. Pierantozzi, Pierluigi Roka, L. Tivani, Martín Torres, Myriam Mariela |
| author_facet | Contreras Valentín, Ana Cibeles Mariotti, Roberto Mousavi, S. Baldoni, L. Guerrero, C. Roka, L. Cultrera, N. Pierantozzi, Pierluigi Maestri, Damián Gentili, Luciana Tivani, Martín Torres, Myriam Mariela |
| author_sort | Contreras Valentín, Ana Cibeles |
| collection | INTA Digital |
| description | Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic
acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location. |
| format | Artículo |
| id | INTA9283 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA92832021-05-05T19:13:50Z Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development Contreras Valentín, Ana Cibeles Mariotti, Roberto Mousavi, S. Baldoni, L. Guerrero, C. Roka, L. Cultrera, N. Pierantozzi, Pierluigi Maestri, Damián Gentili, Luciana Tivani, Martín Torres, Myriam Mariela Olea europaea Expresión Génica Mesocarpio Gene Expression Mesocarp Genetics Genética Olivo Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location. EEA San Juan Fil: Contreras Valetín, Ana Cibeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Contreras Valetín, Ana Cibeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mariotti, Roberto. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia Fil: Mousavi, S. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia Fil: Baldoni, L. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia Fil: Guerrero, C. University of Malaga. Science Faculty. Department of Molecular Biology and Biochemistry ; España Fil: Roka, L. Agricultural University of Athens. Department of Biotechnology; Grecia Fil: Cultrera, N. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia Fil: Pierantozzi, Pierluigi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Pierantozzi, Pierluigi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maestri, Damián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Maestri, Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gentili, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Gentili, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tivani, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Tivani, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina. Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-05-05T18:56:59Z 2021-05-05T18:56:59Z 2020-05-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9283 https://link.springer.com/article/10.1007/s11033-020-05554-9 0301-4851 1573-4978 https://doi.org/10.1007/s11033-020-05554-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Molecular Biology Reports 47 (6) : 4345-4355 (May 2020) |
| spellingShingle | Olea europaea Expresión Génica Mesocarpio Gene Expression Mesocarp Genetics Genética Olivo Contreras Valentín, Ana Cibeles Mariotti, Roberto Mousavi, S. Baldoni, L. Guerrero, C. Roka, L. Cultrera, N. Pierantozzi, Pierluigi Maestri, Damián Gentili, Luciana Tivani, Martín Torres, Myriam Mariela Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title_full | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title_fullStr | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title_full_unstemmed | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title_short | Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development |
| title_sort | characterization and validation of olive fad and sad gene families expression analysis in diferent tissues and during fruit development |
| topic | Olea europaea Expresión Génica Mesocarpio Gene Expression Mesocarp Genetics Genética Olivo |
| url | http://hdl.handle.net/20.500.12123/9283 https://link.springer.com/article/10.1007/s11033-020-05554-9 https://doi.org/10.1007/s11033-020-05554-9 |
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