In vivo neutralization of bee venom lethality by IgY antibodies
Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize...
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9280 https://www.sciencedirect.com/science/article/pii/S0161589021001322 https://doi.org/10.1016/j.molimm.2021.04.015 |
| _version_ | 1855484365229785088 |
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| author | Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo |
| author_browse | Brero, Luisa Cangelosi, Adriana Celi Castillo, Ana Beatriz Chacana, Pablo Geoghegan, Patricia Lammer, Mónica Leiva, Carlos Leónidas Mariconda, Virginia |
| author_facet | Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo |
| author_sort | Leiva, Carlos Leónidas |
| collection | INTA Digital |
| description | Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings. |
| format | Artículo |
| id | INTA9280 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA92802021-05-05T16:54:26Z In vivo neutralization of bee venom lethality by IgY antibodies Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY Bee venom is a complex mixture of molecules, among which melittin and phospholipase A2 (PLA2) are the toxic components involved in envenoming accidents with multiple honeybee stings. Traditionally, the treatment of envenomings has been based on the administration of specific antibodies to neutralize the deleterious effects of toxins. An alternative to mammalian polyclonal antibodies is the use of egg yolk immunoglobulins (IgY) due to their advantages regarding animal welfare and lower costs of production as compared to the conventional production methods. In this work, a novel composition containing specific IgY antibodies was developed. After four immunizations, IgY extracted from the egg yolks was able to recognize several components of the bee venom, including melittin and PLA2. The performance of IgY to neutralize the lethal activity was evaluated in a mouse model by using one median lethal dose (LD50) of the bee venom. The effective dose of the IgY extract was determined as 30.66 μg/mg. These results demonstrate the feasibility to produce IgY-based antivenoms to treat envenomings by multiple bee stings. Instituto de Patobiología Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Geoghegan, Patricia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Lammer, Mónica. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Cangelosi, Adriana. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Mariconda, Virginia. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Celi Castillo, Ana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Celi Castillo, Ana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brero, Luisa. Ministerio de Salud. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán”. Centro Nacional de Control de Calidad de Biológicos; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-05-05T16:38:06Z 2021-05-05T16:38:06Z 2021-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9280 https://www.sciencedirect.com/science/article/pii/S0161589021001322 1872-9142 https://doi.org/10.1016/j.molimm.2021.04.015 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Molecular Immunology 135 : 183-190 (Julio 2021) |
| spellingShingle | Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY Leiva, Carlos Leónidas Geoghegan, Patricia Lammer, Mónica Cangelosi, Adriana Mariconda, Virginia Celi Castillo, Ana Beatriz Brero, Luisa Chacana, Pablo In vivo neutralization of bee venom lethality by IgY antibodies |
| title | In vivo neutralization of bee venom lethality by IgY antibodies |
| title_full | In vivo neutralization of bee venom lethality by IgY antibodies |
| title_fullStr | In vivo neutralization of bee venom lethality by IgY antibodies |
| title_full_unstemmed | In vivo neutralization of bee venom lethality by IgY antibodies |
| title_short | In vivo neutralization of bee venom lethality by IgY antibodies |
| title_sort | in vivo neutralization of bee venom lethality by igy antibodies |
| topic | Apis mellifera Veneno de Abeja Anticuerpos Yema de Huevo Experimentación in Vivo Efectos Letales Neutralización Bee Venoms Antibodies Egg Yolk In vivo Experimentation Lethal Effects Neutralization IgY Antibodies Anticuerpos IgY |
| url | http://hdl.handle.net/20.500.12123/9280 https://www.sciencedirect.com/science/article/pii/S0161589021001322 https://doi.org/10.1016/j.molimm.2021.04.015 |
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