Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells
Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to se...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/919 http://www.sciencedirect.com/science/article/pii/S0378113515301085?via%3Dihub https://doi.org/10.1016/j.vetmic.2015.12.002 |
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| author | Pereyra, Elizabet Amanda Lorena Renna, María Sol Picech, Florencia Baravalle, Celina Andreotti, Carolina Soledad Russi, Romina Calvinho, Luis Fernando Diez, Cristina Dallard, Bibiana Elisabet |
| author_browse | Andreotti, Carolina Soledad Baravalle, Celina Calvinho, Luis Fernando Dallard, Bibiana Elisabet Diez, Cristina Pereyra, Elizabet Amanda Lorena Picech, Florencia Renna, María Sol Russi, Romina |
| author_facet | Pereyra, Elizabet Amanda Lorena Renna, María Sol Picech, Florencia Baravalle, Celina Andreotti, Carolina Soledad Russi, Romina Calvinho, Luis Fernando Diez, Cristina Dallard, Bibiana Elisabet |
| author_sort | Pereyra, Elizabet Amanda Lorena |
| collection | INTA Digital |
| description | Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the behavior of these strains in contact with bovine mammary epithelial cells (MAC-T) and the expression of those genes during bacterial-cell early interactions. The genetic diversity of 20 S. aureus strains that were isolated from milk samples taken from cows with persistent-P and non-persistent-NP IMI was high, discriminated into 13 fingerprint groups. The occurrence of genes coding for S. aureus surface proteins (clfA, clfB, fnbA, fnbB, fib, cna) and biofilm formation (icaA, icaD, icaC, bap) and in vitro biofilm-forming ability was not related to strain clinical origin (NP or P). Internalization of S. aureus into MAC-T cells was strain-dependent and internalized bacteria overexpressed adherence and biofilm-forming genes compared with those that remained in the supernatant of co-cultures; particularly those genes encoding FnBPs and IcaD. Strains yielding highest invasion percentages were those able to overexpress fnBP, irrespectively of the presence of other evaluated genes. Strains from NP IMI showed a greater multiplication capacity in vitro compared with strains from P IMI. These results provide new insights about S. aureus differential gene expression of adhesion-internalization factors during early interaction with mammary epithelial cells. |
| format | Artículo |
| id | INTA919 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9192019-03-22T12:41:13Z Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells Pereyra, Elizabet Amanda Lorena Renna, María Sol Picech, Florencia Baravalle, Celina Andreotti, Carolina Soledad Russi, Romina Calvinho, Luis Fernando Diez, Cristina Dallard, Bibiana Elisabet Staphylococcus aureus Genética Mastitis Enfermedades de los Animales Genetics Animal Diseases Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the behavior of these strains in contact with bovine mammary epithelial cells (MAC-T) and the expression of those genes during bacterial-cell early interactions. The genetic diversity of 20 S. aureus strains that were isolated from milk samples taken from cows with persistent-P and non-persistent-NP IMI was high, discriminated into 13 fingerprint groups. The occurrence of genes coding for S. aureus surface proteins (clfA, clfB, fnbA, fnbB, fib, cna) and biofilm formation (icaA, icaD, icaC, bap) and in vitro biofilm-forming ability was not related to strain clinical origin (NP or P). Internalization of S. aureus into MAC-T cells was strain-dependent and internalized bacteria overexpressed adherence and biofilm-forming genes compared with those that remained in the supernatant of co-cultures; particularly those genes encoding FnBPs and IcaD. Strains yielding highest invasion percentages were those able to overexpress fnBP, irrespectively of the presence of other evaluated genes. Strains from NP IMI showed a greater multiplication capacity in vitro compared with strains from P IMI. These results provide new insights about S. aureus differential gene expression of adhesion-internalization factors during early interaction with mammary epithelial cells. EEA Rafaela Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Renna, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Picech, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Andreotti, Carolina S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Russi, Romina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Diez, Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina 2017-08-08T12:32:06Z 2017-08-08T12:32:06Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/919 http://www.sciencedirect.com/science/article/pii/S0378113515301085?via%3Dihub 0378-1135 https://doi.org/10.1016/j.vetmic.2015.12.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary microbiology 183 : 69-77. (2016) |
| spellingShingle | Staphylococcus aureus Genética Mastitis Enfermedades de los Animales Genetics Animal Diseases Pereyra, Elizabet Amanda Lorena Renna, María Sol Picech, Florencia Baravalle, Celina Andreotti, Carolina Soledad Russi, Romina Calvinho, Luis Fernando Diez, Cristina Dallard, Bibiana Elisabet Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title | Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title_full | Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title_fullStr | Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title_full_unstemmed | Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title_short | Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells |
| title_sort | detection of staphylococcus aureus adhesion and biofilm producing genes and their expression during internalization in bovine mammary epithelial cells |
| topic | Staphylococcus aureus Genética Mastitis Enfermedades de los Animales Genetics Animal Diseases |
| url | http://hdl.handle.net/20.500.12123/919 http://www.sciencedirect.com/science/article/pii/S0378113515301085?via%3Dihub https://doi.org/10.1016/j.vetmic.2015.12.002 |
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